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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1991-1-11
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pubmed:abstractText |
G0 human T cells synthesize protein at low rates and contain very low levels of eIF-2 alpha mRNA. eIF-2 alpha plays a pivotal role in the earliest regulated steps of translation initiation. We examined eIF-2 alpha gene expression in normal human T cells stimulated with PHA. Nuclear run-on assays indicate low rates of eIF-2 alpha gene transcription in G0 cells and these change 2-fold with PHA treatment. Actinomycin D chase experiments show that the t1/2 of eIF-2 alpha mRNA is similar in G0 and PHA-treated T cells. Analysis of nuclear RNA with probes specific for eIF-2 alpha intron sequences shows that increased eIF-2 alpha expression after PHA treatment is largely due to intranuclear stabilization of the primary transcript. The increase in eIF-2 alpha mRNA does not require new protein synthesis. Hence, expression of this gene appears to be a part of the primary response program of T cells when they are exposed to mitogen.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-1078787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-1132441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-1268258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-200446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2443809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2498643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2542864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2582137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2672437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2783497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2806919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2825027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2842344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2898781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2910496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2948954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2978906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-2989408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3192523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3265337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3499567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3520340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3603668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-3823900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-465497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-4824348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-4836354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-510295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-5505329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-591563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6164076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6171820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6195151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6205392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-623765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6492167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-668908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6736126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6750132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6910478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6937266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-6970214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-7365257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-7409177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701139-7461126
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3831-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1701139-Cell Nucleus,
pubmed-meshheading:1701139-Cells, Cultured,
pubmed-meshheading:1701139-Eukaryotic Initiation Factor-2,
pubmed-meshheading:1701139-Guanosine Triphosphate,
pubmed-meshheading:1701139-Humans,
pubmed-meshheading:1701139-Kinetics,
pubmed-meshheading:1701139-Lymphocyte Activation,
pubmed-meshheading:1701139-Phytohemagglutinins,
pubmed-meshheading:1701139-RNA,
pubmed-meshheading:1701139-RNA, Messenger,
pubmed-meshheading:1701139-T-Lymphocytes,
pubmed-meshheading:1701139-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1701139-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Increased eIF-2 alpha expression in mitogen-activated primary T lymphocytes.
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pubmed:affiliation |
Laboratory of Molecular Hematology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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