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pubmed-article:17011005pubmed:abstractTextSpecies identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL.lld:pubmed
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pubmed-article:17011005pubmed:articleTitleAmerican tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples.lld:pubmed
pubmed-article:17011005pubmed:affiliationLaboratory of Molecular Parasitology, Instituut voor Tropische Geneeskunde, Nationalestraat 155, B-2000 Antwerpen, Belgium.lld:pubmed
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