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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
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|
pubmed:dateCreated |
1990-12-5
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|
pubmed:abstractText |
A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
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pubmed:issn |
0006-291X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
30
|
|
pubmed:volume |
172
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
511-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1700704-Base Sequence,
pubmed-meshheading:1700704-Blotting, Western,
pubmed-meshheading:1700704-Cloning, Molecular,
pubmed-meshheading:1700704-Escherichia coli,
pubmed-meshheading:1700704-Genes, Viral,
pubmed-meshheading:1700704-Hepacivirus,
pubmed-meshheading:1700704-Hepatitis C,
pubmed-meshheading:1700704-Humans,
pubmed-meshheading:1700704-Molecular Sequence Data,
pubmed-meshheading:1700704-Oligonucleotide Probes,
pubmed-meshheading:1700704-Plasmids,
pubmed-meshheading:1700704-RNA, Viral,
pubmed-meshheading:1700704-RNA-Directed DNA Polymerase,
pubmed-meshheading:1700704-Viral Structural Proteins
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|
pubmed:year |
1990
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|
pubmed:articleTitle |
A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus.
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pubmed:affiliation |
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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