|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2007-9-27
|
|
pubmed:abstractText |
Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5 kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5 kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20 degrees C. We conclude that the use of the 12.5 kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0364-5134
|
|
pubmed:author |
pubmed-author:BernardiniSergioS,
pubmed-author:D'AguannoSimonaS,
pubmed-author:Del BoccioPieroP,
pubmed-author:Di IlioCarmineC,
pubmed-author:Di IoiaMariaM,
pubmed-author:FedericiGiorgioG,
pubmed-author:GambiDomenicoD,
pubmed-author:LugaresiAlessandraA,
pubmed-author:PavoneBarbaraB,
pubmed-author:PieragostinoDamianaD,
pubmed-author:SacchettaPaoloP,
pubmed-author:TravagliniDanielaD,
pubmed-author:UrbaniAndreaA
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
201-4; discussion 205
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:17006926-Artifacts,
pubmed-meshheading:17006926-Cystatin C,
pubmed-meshheading:17006926-Cystatins,
pubmed-meshheading:17006926-Drug Storage,
pubmed-meshheading:17006926-Freezing,
pubmed-meshheading:17006926-Humans,
pubmed-meshheading:17006926-Molecular Weight,
pubmed-meshheading:17006926-Multiple Sclerosis,
pubmed-meshheading:17006926-Peptide Fragments,
pubmed-meshheading:17006926-Spectrometry, Mass, Matrix-Assisted Laser...
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Cleavage of cystatin C is not associated with multiple sclerosis.
|
|
pubmed:affiliation |
Centro Studi sull'Invecchiamento, Fondazione G. D'Annunzio, Chieti, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
