Linked Life Data

17005908

Source:http://linkedlifedata.com/resource/pubmed/id/17005908

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-9-28
pubmed:abstractText
The functions of gammadelta T cells are enigmatic, and these cells are often considered as evolutionary remnants of well-characterized alphabeta T cells. However, their conservation throughout evolution suggests that gammadelta T cells are biologically unique. In ruminants, gammadelta T cells expressing the workshop cluster 1 (WC1) scavenger receptor comprise a large proportion of circulating lymphocytes, suggesting these cells are biologically relevant and functionally different from alphabeta T cells. In fact, bovine WC1(+) gammadelta T cells can act as APC for alphabeta T cells, indicating they may express genes encoding proteins associated with innate immunity. The present study was designed to compare immune function gene expression profiles of clonal populations of WC1(+) gammadelta and CD4(+) alphabeta T cells derived from the same animal, which respond to major surface protein 2 (MSP2) of the intraerythrocytic rickettsial pathogen of cattle, Anaplasma marginale. Gene expression profiles of activated T cell clones were compared using a microarray format, and differential gene expression was confirmed by real-time RT-PCR and protein analyses. We demonstrate that although MSP2-specific alphabeta and gammadelta T cell clones express many of the same genes, gammadelta T cell clones express high levels of genes associated with myeloid cells, including chemokines CCL2, CXCL1, CXCL2, CXCL6, and surface receptors CD68, CD11b, macrophage scavenger receptor 1, macrophage mannose receptor, and galectin-3. It is important that many of these genes were also expressed at higher levels in polyclonal WC1(+) gammadelta T cells when compared with CD4(+) alphabeta T cells selected from peripheral blood.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
939-52
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17005908-Anaplasma marginale, pubmed-meshheading:17005908-Animals, pubmed-meshheading:17005908-CD4-Positive T-Lymphocytes, pubmed-meshheading:17005908-Cattle, pubmed-meshheading:17005908-Cell Line, pubmed-meshheading:17005908-Chemokines, pubmed-meshheading:17005908-Gene Expression Profiling, pubmed-meshheading:17005908-Membrane Glycoproteins, pubmed-meshheading:17005908-Myeloid Cells, pubmed-meshheading:17005908-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17005908-RNA, Messenger, pubmed-meshheading:17005908-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:17005908-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:17005908-Receptors, Cell Surface, pubmed-meshheading:17005908-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17005908-Sensitivity and Specificity, pubmed-meshheading:17005908-T-Lymphocyte Subsets
pubmed:year
2006
pubmed:articleTitle
Comparative gene expression by WC1+ gammadelta and CD4+ alphabeta T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ gammadelta T cells.
pubmed:affiliation
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural