| pubmed-article:17001307 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0019647 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0071216 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0086222 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0597360 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:17001307 | lifeskim:mentions | umls-concept:C1705733 | lld:lifeskim |
| pubmed-article:17001307 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:17001307 | pubmed:dateCreated | 2007-3-29 | lld:pubmed |
| pubmed-article:17001307 | pubmed:abstractText | The transcriptionally regulated urokinase-type plasminogen activator receptor (u-PAR) contributes to cancer progression. Although previous studies have identified multiple 5' regulatory elements, these cis motifs cannot fully account for u-PAR expression prompting a search for hitherto uncharacterized regulatory elements. DNase I hypersensitivity and chromatin immunoprecipitation assays using u-PAR-expressing colon cancer cells indicated a hypersensitive region (+665/+2068) in intron 1 enriched with acetylated histone 3 (H3) and H3 methylated at lysine 4, markers of regulatory regions. The +665/+2068 region increased transcription from a u-PAR-promoter in an orientation- and distance-independent manner fulfilling the criteria of an enhancer. Optimal stimulation of the u-PAR promoter by phorbol ester required this enhancer. Systematic truncations combined with DNase I footprinting revealed two protected regions (+1060/+1099 and +1123/+1134) with deletion of the latter practically abolishing enhancer activity. The +1123/+1134 region harbored non-consensus activator protein-1 and Ets1 binding sites bound with c-Jun (and/or the related JunD/JunB) and c-Fos (and/or the related FosB/Fra-1/Fra-2) as revealed with chromatin immunoprecipitation. Further, nuclear extract from resected colon cancers showed elevated protein binding to a +1123/+1134-spanning probe coordinate with elevated u-PAR protein. Thus, we have defined a novel intragenic enhancer in the u-PAR gene required for constitutive and inducible expression. | lld:pubmed |
| pubmed-article:17001307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17001307 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17001307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:17001307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17001307 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17001307 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17001307 | pubmed:issn | 0950-9232 | lld:pubmed |
| pubmed-article:17001307 | pubmed:author | pubmed-author:WangHH | lld:pubmed |
| pubmed-article:17001307 | pubmed:author | pubmed-author:AllgayerHH | lld:pubmed |
| pubmed-article:17001307 | pubmed:author | pubmed-author:BoydD DDD | lld:pubmed |
| pubmed-article:17001307 | pubmed:author | pubmed-author:YapMM | lld:pubmed |
| pubmed-article:17001307 | pubmed:author | pubmed-author:AsanganiII | lld:pubmed |
| pubmed-article:17001307 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17001307 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:17001307 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:17001307 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17001307 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17001307 | pubmed:pagination | 2058-70 | lld:pubmed |
| pubmed-article:17001307 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
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| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:meshHeading | pubmed-meshheading:17001307... | lld:pubmed |
| pubmed-article:17001307 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17001307 | pubmed:articleTitle | Identification of an histone H3 acetylated/K4-methylated-bound intragenic enhancer regulatory for urokinase receptor expression. | lld:pubmed |
| pubmed-article:17001307 | pubmed:affiliation | Department of Cancer Biology, MD Anderson Cancer Center, Houston, TX 77030, USA. | lld:pubmed |
| pubmed-article:17001307 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17001307 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:5329 | entrezgene:pubmed | pubmed-article:17001307 | lld:entrezgene |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17001307 | lld:pubmed |
