Linked Life Data

17000643

Source:http://linkedlifedata.com/resource/pubmed/id/17000643

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-12-15
pubmed:abstractText
Currently no single proteomics technology has sufficient analytical power to allow for the detection of an entire proteome of an organelle, cell, or tissue. One approach that can be used to expand proteome coverage is the use of multiple separation technologies especially if there is minimal overlap in the proteins observed by the different methods. Using the inner mitochondrial membrane subproteome as a model proteome, we compared for the first time the ability of three protein separation methods (two-dimensional liquid chromatography using the ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter, one-dimensional reversed phase high performance liquid chromatography, and two-dimensional gel electrophoresis) to determine the relative overlap in protein separation for these technologies. Data from these different methods indicated that a strikingly low number of proteins overlapped with less than 24% of proteins common between any two technologies and only 7% common among all three methods. Utilizing the three technologies allowed the creation of a composite database totaling 348 non-redundant proteins. 82% of these proteins had not been observed previously in proteomics studies of this subproteome, whereas 44% had not been identified in proteomics studies of intact mitochondria. Each protein separation method was found to successfully resolve a unique subset of proteins with the liquid chromatography methods being more suited for the analysis of transmembrane domain proteins and novel protein discovery. We also demonstrated that both the one- and two-dimensional LC allowed for the separation of the alpha-subunit of F1F0 ATP synthase that differed due to a change in pI or hydrophobicity.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/CA10951, http://linkedlifedata.com/resource/pubmed/grant/N0-HV-28180, http://linkedlifedata.com/resource/pubmed/grant/P01 HL077180-010001, http://linkedlifedata.com/resource/pubmed/grant/P01 HL077180-020001, http://linkedlifedata.com/resource/pubmed/grant/P01 HL077180-030001, http://linkedlifedata.com/resource/pubmed/grant/P01 HL077180-040001, http://linkedlifedata.com/resource/pubmed/grant/P01 HL077180-050001, http://linkedlifedata.com/resource/pubmed/grant/P01 HL081427-010003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL081427-020003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL081427-030003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL081427-040003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL081427-050003, http://linkedlifedata.com/resource/pubmed/grant/P50 HL084946-019003, http://linkedlifedata.com/resource/pubmed/grant/P50 HL084946-029003, http://linkedlifedata.com/resource/pubmed/grant/P50 HL084946-039003
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1535-9476
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2392-411
pubmed:dateRevised
2010-8-9
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Expanding the subproteome of the inner mitochondria using protein separation technologies: one- and two-dimensional liquid chromatography and two-dimensional gel electrophoresis.
pubmed:affiliation
Department of Medicine, The Technical Implementation and Coordination Core of The Johns Hopkins NHLBI Proteomics Center, The Johns Hopkins University, Baltimore, MD 21224, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural