Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1990-12-27
|
| pubmed:abstractText |
Meningioma is a generally benign tumor derived from arachnoid tissue. We have investigated the presence of functionally active PDGF-receptors on human meningioma cells in culture. Tumor samples were obtained from 3 surgically removed benign meningiomas and normal arachnoid tissue from an autopsy case. Binding studies were performed by using 125I-labelled recombinant PDGF-AA and PDGF-BB. Only 125I-PDGF-BB showed specific binding to all tumor-cell cultures after incubation of cells for 2 hr at 4 degrees C. Effects of PDGF-AA and PDGF-BB on DNA synthesis were measured as 3H-thymidine incorporation during 48 hr of labelling cells maintained in Eagle's minimum essential medium 0.5% fetal calf serum. PDGF-BB but not PDGF-AA stimulated DNA synthesis in all 3 tumor-cell cultures. Total cellular RNA was analyzed by Northern blotting and hybridization with a 32P-labelled human PDGF beta-receptor probe, and PDGF beta-receptor mRNA was found in both tumor and arachnoid cell cultures. Furthermore, PDGF beta-receptor mRNA was shown to be present in 2 meningioma biopsies and immunohistochemical staining revealed that PDGF beta-receptors are present in meningioma and arachnoid tissues in vivo. It appears that a possible way of maintaining human meningioma cell growth in vivo is through activation of PDGF beta-receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Platelet-Derived Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
772-8
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:1699901-Adult,
pubmed-meshheading:1699901-Aged,
pubmed-meshheading:1699901-Arachnoid,
pubmed-meshheading:1699901-Blotting, Northern,
pubmed-meshheading:1699901-Cells, Cultured,
pubmed-meshheading:1699901-Female,
pubmed-meshheading:1699901-Humans,
pubmed-meshheading:1699901-Immunohistochemistry,
pubmed-meshheading:1699901-Iodine Radioisotopes,
pubmed-meshheading:1699901-Male,
pubmed-meshheading:1699901-Meningioma,
pubmed-meshheading:1699901-Platelet-Derived Growth Factor,
pubmed-meshheading:1699901-RNA, Messenger,
pubmed-meshheading:1699901-Receptors, Cell Surface,
pubmed-meshheading:1699901-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:1699901-Staining and Labeling,
pubmed-meshheading:1699901-Thymidine,
pubmed-meshheading:1699901-Tritium,
pubmed-meshheading:1699901-Tumor Cells, Cultured
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Expression of PDGF beta-receptors in human meningioma cells.
|
| pubmed:affiliation |
Department of Pathology, University Hospital, Uppsala, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|