Linked Life Data

16997211

Source:http://linkedlifedata.com/resource/pubmed/id/16997211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-9-25
pubmed:abstractText
A method of fluorescence anisotropy decay analysis is described in this work. The transient anisotropy r(ex)(t) measured in a photocounting pulsefluorimeter is fitted by a non linear least square procedure to the ratio of convolutions of the apparatus response function g(t) by sums of appropriate exponential functions. This method takes rigorously into account the apparatus response function and is applicable to any shape of the later as well as to any values of fluorescence decay times and correlation times. The performances of the method have been tested with data simulated from measured response functions corresponding to an air lamp and a high pressure nitrogen lamp. The statistical standard errors of the anisotropy deca parameters have been found to be smaller than the standard errors previously calculated for the moment method. A systematic error delta in the fluorescence decay time entailed an error deltatheta in the correlation time such as Deltatheta/theta < deltatau/tau. By this method, good fitting of experimental data have been achieved very conveniently and accurately.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jul
pubmed:issn
0301-4622
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-104
pubmed:year
1979
pubmed:articleTitle
Analysis of fluorescence anisotropy decays by a least square method.
pubmed:affiliation
Centre de Biophysique Moléculaire, C.N.R.S., 45045 Orleans Cedex, France.
pubmed:publicationType
Journal Article