Source:http://linkedlifedata.com/resource/pubmed/id/16995780
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2006-9-25
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| pubmed:abstractText |
Various cell delivery methods have been investigated for cell transplantation treatment of cardiac infarcts. In this study, we investigated a type I collagen-glycosaminoglycan (GAG) scaffold for the implantation of adult bone marrow-derived mesenchymal stem cells (MSCs) into the infarcted region in the rat heart. The objective was to evaluate the tissue response to collagen-GAG scaffolds prepared using 2 cross-linking methods. The left coronary artery of female Wistar rats was occluded for 60 min, followed by reperfusion. One week later, the infarcted region was implanted with (1) collagen-GAG scaffolds cross-linked by dehydrothermal treatment alone (DHT; n = 10); (2) collagen-GAG scaffolds cross-linked by DHT followed by carbodiimide treatment (EDAC; n = 8); or (3) DHT cross-linked collagen-GAG scaffolds seeded with bromodeoxyuridine (BrdU)-labeled allogeneic MSCs (cell-scaffold; n = 9). Shamoperated rats served as controls (n = 4). Specimens were harvested 3 weeks after the implantation surgery. The tissue response was evaluated histomorphometrically and by immunohistochemistry to track the BrdU-labeled MSCs. Most of the DHT cross-linked collagen-GAG scaffolds degraded, whereas the scaffolds in the EDAC group appeared to be largely intact. There were no signs of acute inflammation in any of the groups. A substantial amount of neovascularization was seen in the infarcted region in the implant groups and in the scaffolds themselves. BrdU-positive cells appeared both in the degraded scaffold and the infarct region. DHT cross-linked collagen-GAG scaffolds warrant continued investigation as delivery vehicles for implantation of cells into infarcted cardiac tissue.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
1076-3279
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2467-78
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16995780-Absorbable Implants,
pubmed-meshheading:16995780-Animals,
pubmed-meshheading:16995780-Collagen,
pubmed-meshheading:16995780-Female,
pubmed-meshheading:16995780-Glycosaminoglycans,
pubmed-meshheading:16995780-Male,
pubmed-meshheading:16995780-Mesenchymal Stem Cell Transplantation,
pubmed-meshheading:16995780-Mesenchymal Stem Cells,
pubmed-meshheading:16995780-Models, Cardiovascular,
pubmed-meshheading:16995780-Myocardial Infarction,
pubmed-meshheading:16995780-Myocardial Reperfusion Injury,
pubmed-meshheading:16995780-Myocardium,
pubmed-meshheading:16995780-Neovascularization, Physiologic,
pubmed-meshheading:16995780-Rats,
pubmed-meshheading:16995780-Rats, Wistar,
pubmed-meshheading:16995780-Tissue Engineering,
pubmed-meshheading:16995780-Transplantation, Homologous
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Collagen-GAG scaffolds grafted onto myocardial infarcts in a rat model: a delivery vehicle for mesenchymal stem cells.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Huaxi Hospital, Sichuan University, Chengdu, China.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|