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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-11-7
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pubmed:abstractText |
The interaction of ruthenium red, [(NH3)5Ru-O-Ru(NH3)4-O-Ru(NH3)5]Cl6.4H2O, with various Ca2(+)-binding proteins was studied. Ruthenium red inhibited Ca2+ binding to the sarcoplasmic reticulum protein, calsequestrin, immobilized on Sepharose 4B. Furthermore, ruthenium red bound to calsequestrin with high affinity (Kd = 0.7 microM; Bmax = 218 nmol/mg protein). The dye stained calsequestrin in sodium dodecyl sulfate-polyacrylamide gels or on nitrocellulose paper and was displaced by Ca2+ (Ki = 1.4 mM). The specificity of ruthenium red staining of several Ca2(+)-binding proteins was investigated by comparison with two other detection methods, 45Ca2+ autoradiography and the Stains-all reaction. Ruthenium red bound to the same proteins detected by the 45Ca2+ overlay technique. Ruthenium red stained both the erythrocyte Band 3 anion transporter and the Ca2(+)-ATPase of skeletal muscle sarcoplasmic reticulum. Ruthenium red also stained the EF hand conformation Ca2(+)-binding proteins, calmodulin, troponin C, and S-100. This inorganic dye provides a simple, rapid method for detecting various types of Ca2(+)-binding proteins following electrophoresis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calsequestrin,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Red
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
188
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1699445-Animals,
pubmed-meshheading:1699445-Calcium,
pubmed-meshheading:1699445-Calcium Radioisotopes,
pubmed-meshheading:1699445-Calcium-Binding Proteins,
pubmed-meshheading:1699445-Calsequestrin,
pubmed-meshheading:1699445-Dogs,
pubmed-meshheading:1699445-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1699445-Erythrocytes,
pubmed-meshheading:1699445-Humans,
pubmed-meshheading:1699445-Immunoblotting,
pubmed-meshheading:1699445-Muscles,
pubmed-meshheading:1699445-Protein Binding,
pubmed-meshheading:1699445-Rabbits,
pubmed-meshheading:1699445-Ruthenium Red,
pubmed-meshheading:1699445-Sarcoplasmic Reticulum,
pubmed-meshheading:1699445-Staining and Labeling
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pubmed:year |
1990
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pubmed:articleTitle |
Interaction of ruthenium red with Ca2(+)-binding proteins.
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pubmed:affiliation |
MRC Group in Membrane Biology, Department of Medicine, University of Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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