|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
48
|
|
pubmed:dateCreated |
2006-11-27
|
|
pubmed:abstractText |
We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP(3)R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP(3)R. DANGER interacts with IP(3)R in vitro and co-immunoprecipitates with IP(3)R from cellular preparations. DANGER robustly enhances Ca(2+)-mediated inhibition of IP(3) RCa(2+) release without affecting IP(3) binding in microsomal assays and inhibits gating in single-channel recordings of IP(3)R. DANGER appears to allosterically modulate the sensitivity of IP(3) RtoCa(2+) inhibition, which likely alters IP(3)R-mediated Ca(2+) dynamics in cells where DANGER and IP(3)R are co-expressed.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
281
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
37111-6
|
|
pubmed:dateRevised |
2008-9-13
|
|
pubmed:meshHeading |
pubmed-meshheading:16990268-Allosteric Site,
pubmed-meshheading:16990268-Animals,
pubmed-meshheading:16990268-Calcium,
pubmed-meshheading:16990268-Cloning, Molecular,
pubmed-meshheading:16990268-Electrophysiology,
pubmed-meshheading:16990268-Gene Expression Regulation,
pubmed-meshheading:16990268-Humans,
pubmed-meshheading:16990268-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:16990268-Insects,
pubmed-meshheading:16990268-Membrane Proteins,
pubmed-meshheading:16990268-Neurons,
pubmed-meshheading:16990268-Protein Binding,
pubmed-meshheading:16990268-Protein Structure, Tertiary,
pubmed-meshheading:16990268-Rats,
pubmed-meshheading:16990268-Trypsin,
pubmed-meshheading:16990268-Two-Hybrid System Techniques
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity.
|
|
pubmed:affiliation |
Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
