pubmed-article:1698880 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0205282 | lld:lifeskim |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:1698880 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
pubmed-article:1698880 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:1698880 | pubmed:dateCreated | 1990-11-20 | lld:pubmed |
pubmed-article:1698880 | pubmed:abstractText | Although many different protocols for transfection of lymphoid cell lines exist, successful DNA transfer into primary B lymphocytes has, to date, not been demonstrated. We now describe a simple method for gene transfer into highly purified normal and malignant B lymphocytes by electroporation. Using a powerful expression vector containing two copies of the cytomegalovirus (CMV) immediate early enhancer linked to the human T cell lymphotropic virus I (HTLV I) promoter, we could demonstrate transfected gene expression in both high density small 'resting' B cells and in low density 'activated' B cells. Successful transfection was detected by expression of chloramphenicol acetyl transferase and by immunofluorescence. The neoplastic cells of B cell chronic lymphocytic leukemia could also be transfected with an efficiency of 5-10%, but only after preactivation. This method of transfection will permit analysis of the contribution of individual genes and their products to normal and malignant B cell growth and differentiation. | lld:pubmed |
pubmed-article:1698880 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1698880 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1698880 | pubmed:language | eng | lld:pubmed |
pubmed-article:1698880 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1698880 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1698880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1698880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:1698880 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1698880 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1698880 | pubmed:month | Oct | lld:pubmed |
pubmed-article:1698880 | pubmed:issn | 0022-1759 | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:BrennerM KMK | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:ChenI SIS | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:RooneyC MCM | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:BuschleMM | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:DrexlerH GHG | lld:pubmed |
pubmed-article:1698880 | pubmed:author | pubmed-author:GignacS MSM | lld:pubmed |
pubmed-article:1698880 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1698880 | pubmed:day | 4 | lld:pubmed |
pubmed-article:1698880 | pubmed:volume | 133 | lld:pubmed |
pubmed-article:1698880 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1698880 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1698880 | pubmed:pagination | 77-85 | lld:pubmed |
pubmed-article:1698880 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
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pubmed-article:1698880 | pubmed:year | 1990 | lld:pubmed |
pubmed-article:1698880 | pubmed:articleTitle | Transfection and gene expression in normal and malignant primary B lymphocytes. | lld:pubmed |
pubmed-article:1698880 | pubmed:affiliation | Ludwig Institute for Cancer Research, St. Mary's Branch, London, U.K. | lld:pubmed |
pubmed-article:1698880 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1698880 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:1698880 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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