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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-10-11
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pubmed:abstractText |
The interpretation of low-level or non-reproducible amplification results in clinical quantitative polymerase chain reaction (Q-PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low-level results might create prognostic or therapeutic dilemmas. We evaluated low-level, ambiguous Q-PCR results in a study of paired diagnostic and end-induction (day 29) TEL-AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low-level apparent Q-PCR positive results may be spurious or non-specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
358-61
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16984387-Child,
pubmed-meshheading:16984387-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:16984387-DNA, Neoplasm,
pubmed-meshheading:16984387-DNA Primers,
pubmed-meshheading:16984387-Flow Cytometry,
pubmed-meshheading:16984387-Fluorescent Dyes,
pubmed-meshheading:16984387-Humans,
pubmed-meshheading:16984387-Neoplasm, Residual,
pubmed-meshheading:16984387-Neoplasm Proteins,
pubmed-meshheading:16984387-Oncogene Proteins, Fusion,
pubmed-meshheading:16984387-Polymerase Chain Reaction,
pubmed-meshheading:16984387-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:16984387-Reproducibility of Results
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pubmed:year |
2006
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pubmed:articleTitle |
Resolution of ambiguous low-level positive quantitative polymerase chain reaction results in TEL-AML1 positive ALL using a post-PCR fluorescent oligoligation method.
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pubmed:affiliation |
Department of Pathology, University of New Mexico HSC and Cancer Research and Treatment Center, Albuquerque, NM USA. ichen@salud.unm.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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