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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0024432,
umls-concept:C0031621,
umls-concept:C0038952,
umls-concept:C0056912,
umls-concept:C0591833,
umls-concept:C1423633,
umls-concept:C1515877,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C1879547
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pubmed:issue |
7
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pubmed:dateCreated |
2006-9-19
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pubmed:abstractText |
Bacterial CpG-containing (CpG) DNA promotes survival of murine macrophages and triggers production of proinflammatory mediators. The CpG DNA-induced inflammatory response is mediated via TLR9, whereas a recent study reported that activation of the Akt prosurvival pathway occurs via DNA-dependent protein kinase (DNA-PK) and independently of TLR9. We show, in this study, that Akt activation and survival of murine bone marrow-derived macrophages (BMM) triggered by CpG-containing phosphodiester oligodeoxynucleotides or CpG-containing phosphorothioate oligodeoxynucleotides was completely dependent on TLR9. In addition, survival triggered by CpG-containing phosphodiester oligodeoxynucleotides was not compromised in BMM from SCID mice that express a catalytically inactive form of DNA-PK. CpG DNA-induced survival of BMM was inhibited by the PI3K inhibitor, LY294002, but not by the MEK1/2 inhibitor, PD98059. The effect of LY294002 was specific to survival, because treatment of BMM with LY294002 affected CpG DNA-induced TNF-alpha production only modestly. Therefore, CpG DNA activates macrophage survival via TLR9 and the PI3K-Akt pathway and independently of DNA-PK and MEK-ERK.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz...,
http://linkedlifedata.com/resource/pubmed/chemical/Chromones,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Morpholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 9,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4473-80
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16982883-Animals,
pubmed-meshheading:16982883-Bone Marrow Cells,
pubmed-meshheading:16982883-Cell Survival,
pubmed-meshheading:16982883-Cells, Cultured,
pubmed-meshheading:16982883-Chromones,
pubmed-meshheading:16982883-CpG Islands,
pubmed-meshheading:16982883-DNA,
pubmed-meshheading:16982883-Enzyme Inhibitors,
pubmed-meshheading:16982883-Flow Cytometry,
pubmed-meshheading:16982883-Immunoblotting,
pubmed-meshheading:16982883-Macrophage Activation,
pubmed-meshheading:16982883-Macrophages,
pubmed-meshheading:16982883-Mice,
pubmed-meshheading:16982883-Morpholines,
pubmed-meshheading:16982883-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:16982883-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:16982883-Toll-Like Receptor 9,
pubmed-meshheading:16982883-Tumor Necrosis Factor-alpha
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pubmed:year |
2006
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pubmed:articleTitle |
CpG DNA activates survival in murine macrophages through TLR9 and the phosphatidylinositol 3-kinase-Akt pathway.
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pubmed:affiliation |
Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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