Linked Life Data

16981195

Source:http://linkedlifedata.com/resource/pubmed/id/16981195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-12-4
pubmed:abstractText
In this work, we compared the performance of objectives with similar numerical aperture of 0.75 but different immersion media of air, water, glycerin, and oil in the imaging of human skin epithelium and dermis. In general, we found that the oil immersion objective recorded the strongest intensity at the same mechanical depth. We also characterized the focal shifts and found that with decreasing refractive index, the focal shift becomes increasingly more negative (for both the epithelium and dermis). In imaging the dermis, we estimated the image resolution at the depths of 18.8 and 30.2 microm, and found that the image resolution were comparable at these depths under the four types of immersion conditions. Our results demonstrate that by changing the immersion media, the main microscopic imaging effects are the recorded axial intensities and the focal shifts. The effects on the image resolution are negligible.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1059-910X
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
992-7
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Effects of different immersion media in multiphoton imaging of the epithelium and dermis of human skin.
pubmed:affiliation
Department of Electrical Engineering, Institute of Electro Optics, National Taiwan University, and Department of Dermatology, National Taiwan University Hospital, Taipei 106, Taiwan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't