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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
25
|
| pubmed:dateCreated |
1990-10-9
|
| pubmed:abstractText |
We have isolated four insulin-like growth factor binding proteins (IGFBPs) from adult human serum by insulin-like growth factor (IGF) I affinity chromatography and high performance liquid chromatography. A 36-kDa binding protein (BP), not digestible with N-glycanase, is increased in patients with extrapancreatic tumor hypoglycemia and during IGF I administration in healthy adults. Its 38 NH2-terminal amino acids are identical to those of an IGFBP sequence derived from a human cDNA that cross-hybridizes with the rat IGFBP-2 cDNA. With probes encoding a NH2-terminal, COOH-terminal, and a middle region of this protein we have obtained three cDNA clones from a Hep G2 cDNA library; one encodes human IGFBP-2, and the other two presumably represent unspliced heteronuclear and alternatively spliced mRNA, respectively. A 28-30-kDa IGFBP represents a novel BP species in human serum. Its 30 NH2-terminal amino acids are not homologous to IGFBP-1, -2, or -3. It is not digestible with N-glycanase and does not bind 125I-IGF I. The NH2-terminal sequences of a 42/45- and a 31-kDa IGFBP are identical to that of human IGFBP-3. The 42/45-kDa proteins are two glycosylation variants of BP-3. The 31-kDa protein presumably is a degradation product of BP-3 that lacks the COOH terminus. It is likely that the different IGFBPs modulate auto-/paracrine and endocrine effects of IGFs on growth and metabolism in a different and specific manner.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Somatomedins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
265
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
14892-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1697583-Adult,
pubmed-meshheading:1697583-Amino Acid Sequence,
pubmed-meshheading:1697583-Animals,
pubmed-meshheading:1697583-Base Sequence,
pubmed-meshheading:1697583-Carcinoma, Hepatocellular,
pubmed-meshheading:1697583-Carrier Proteins,
pubmed-meshheading:1697583-Cell Line,
pubmed-meshheading:1697583-Chromatography, Affinity,
pubmed-meshheading:1697583-Cloning, Molecular,
pubmed-meshheading:1697583-Gene Library,
pubmed-meshheading:1697583-Humans,
pubmed-meshheading:1697583-Hypoglycemia,
pubmed-meshheading:1697583-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:1697583-Insulin-Like Growth Factor I,
pubmed-meshheading:1697583-Ligands,
pubmed-meshheading:1697583-Liver Neoplasms,
pubmed-meshheading:1697583-Molecular Sequence Data,
pubmed-meshheading:1697583-Neoplasms,
pubmed-meshheading:1697583-Oligonucleotide Probes,
pubmed-meshheading:1697583-Plasmids,
pubmed-meshheading:1697583-RNA, Messenger,
pubmed-meshheading:1697583-Reference Values,
pubmed-meshheading:1697583-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1697583-Somatomedins
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia.
|
| pubmed:affiliation |
Department of Medicine, University Hospital, Zurich CH-8091, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|