Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2006-9-15
pubmed:abstractText
The uridylylation of the VPg peptide primer is the first stage in the replication of picornavirus RNA. This process can be achieved in vitro using purified components, including 3B (VPg) with the RNA dependent RNA polymerase (3Dpol), the precursor 3CD, and an RNA template containing the cre/bus. We show that certain RNA sequences within the foot-and-mouth disease virus (FMDV) 5' untranslated region but outside of the cre/bus can enhance VPg uridylylation activity. Furthermore, we have shown that the FMDV 3C protein alone can substitute for 3CD, albeit less efficiently. In addition, the VPg precursors, 3B(3)3C and 3B(123)3C, can function as substrates for uridylylation in the absence of added 3C or 3CD. Residues within the FMDV 3C protein involved in interaction with the cre/bus RNA have been identified and are located on the face of the protein opposite from the catalytic site. These residues within 3C are also essential for VPg uridylylation activity and efficient virus replication.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10466786, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10497078, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10500216, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10573128, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10775595, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-10856256, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-11044080, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-11602738, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-11911365, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12077141, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12097561, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12208947, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12502805, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12525659, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12554882, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12663781, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12692216, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12692218, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12917456, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-12937178, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-1312630, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-14711816, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-14966374, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15314212, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15596823, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15654079, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15858279, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15919922, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15919933, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-15965477, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-16216271, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-16361422, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-16427083, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-16456546, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-6247501, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-7489508, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-7782313, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-8253083, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-8615011, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-8627720, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9032381, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9367789, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9371611, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9658121, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9696813, http://linkedlifedata.com/resource/pubmed/commentcorrection/16973591-9848654
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9865-75
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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