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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1990-9-27
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pubmed:abstractText |
Toxic shock syndrome toxin 1 (TSST-1), a 22-kilodalton protein made by strains of Staphylococcus aureus harboring the chromosomal toxin gene, may elicit toxic shock syndrome in humans. In vitro, TSST-1 induces T cells to proliferate and macrophages to secrete interleukin-1. To conduct a structure-function analysis, point mutations on the TSST-1 gene were generated by site-directed mutagenesis to identify amino acids critical for activity of the toxin. Specific tyrosine and histidine residues were replaced by alanines. Wild-type and mutant TSST-1 gene constructs were expressed in Escherichia coli, and the products were tested for their mitogenic potential and reactivity with a TSST-1 neutralizing monoclonal antibody (MAb 8-5-7). Four of the mutants were similar to the wild type; i.e., the mutant toxins stimulated murine T cells and reacted with MAb 8-5-7 equally as well as the wild type. Two mutants exhibited a decrease in mitogenic activity, but one of these retained the capacity to bind with MAb 8-5-7 while the other was no longer recognized by the same antibody. One double mutant demonstrated minimal mitogenic activity and did not react in enzyme-linked immunosorbent and immunoblot assays with MAb 8-5-7. The data show that specific residues near the carboxy terminus of TSST-1 are essential for mitogenic activity and in forming the epitope recognized by neutralizing MAb 8-5-7.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2313089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2457635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2479030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2499540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2521300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2704927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2731989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2745678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2785644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2830336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2871397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-2931376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3112526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3127526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3132465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3257201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3346079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3570455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3782090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3782792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3871225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3871826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-3981783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6112412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6189784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6332701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6491377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6530509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6631061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696937-6972418
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3020-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1696937-Animals,
pubmed-meshheading:1696937-Antibodies, Monoclonal,
pubmed-meshheading:1696937-Bacterial Toxins,
pubmed-meshheading:1696937-Base Sequence,
pubmed-meshheading:1696937-Binding, Competitive,
pubmed-meshheading:1696937-Blotting, Western,
pubmed-meshheading:1696937-Cloning, Molecular,
pubmed-meshheading:1696937-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1696937-Enterotoxins,
pubmed-meshheading:1696937-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1696937-Epitopes,
pubmed-meshheading:1696937-Escherichia coli,
pubmed-meshheading:1696937-Mice,
pubmed-meshheading:1696937-Mitogens,
pubmed-meshheading:1696937-Molecular Sequence Data,
pubmed-meshheading:1696937-Mutation,
pubmed-meshheading:1696937-Neutralization Tests,
pubmed-meshheading:1696937-Spleen,
pubmed-meshheading:1696937-Superantigens
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pubmed:year |
1990
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pubmed:articleTitle |
Mutants of staphylococcal toxic shock syndrome toxin 1: mitogenicity and recognition by a neutralizing monoclonal antibody.
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pubmed:affiliation |
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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