Linked Life Data

16968708

Source:http://linkedlifedata.com/resource/pubmed/id/16968708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2006-11-6
pubmed:abstractText
Ectopic accumulation of lipid droplets in non-adipose tissues correlates with the degree of insulin resistance in these tissues. Emerging evidence indicates that lipid droplets are specialized organelles that participate in lipid metabolism and intracellular trafficking. These properties are thought to derive from the lipid droplet-associated PAT protein family (perilipin, ADFP, and Tip47). The functions of the ubiquitously distributed adipose differentiation-related protein (ADFP) and Tip47 remain unknown. To evaluate the roles of ADFP and Tip47 in lipid biogenesis and metabolism, ADFP null and wild type (wt) clonal cell lines were established from ADFP null and wt mice, respectively. In ADFP null cells, Tip47 was identified as the sole lipid droplet-associated protein from the PAT family by mass spectroscopy, which was further confirmed by immunoblotting and immunocytochemistry. Following incubation with oleic acid, ADFP null cells were able to form lipid droplets to the same extent as wt cells. No statistical differences between the two cell types were observed in NEFA uptake or lipolysis. Small interference RNAs (siRNAs) against Tip47 were found to down-regulate protein levels for Tip47 by 85%. ADFP null cells treated with Tip47 siRNA retained the ability to form lipid droplets but to a lesser extent and shunted the utilization of exogenously added NEFA from triglycerides to phospholipids. These data support the hypothesis that Tip47 plays an important role in lipid metabolism. Tip47 and ADFP in peripheral tissues may play a critical role in regulating the formation and turnover, and hence metabolic consequences, of ectopic fat.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
34341-8
pubmed:dateRevised
2011-10-18
pubmed:meshHeading
pubmed-meshheading:16968708-Adipocytes, pubmed-meshheading:16968708-Adipose Tissue, pubmed-meshheading:16968708-Animals, pubmed-meshheading:16968708-Cell Differentiation, pubmed-meshheading:16968708-Cell Line, pubmed-meshheading:16968708-Chromatography, Thin Layer, pubmed-meshheading:16968708-Embryo, Mammalian, pubmed-meshheading:16968708-Fibroblasts, pubmed-meshheading:16968708-Immunoblotting, pubmed-meshheading:16968708-Immunoenzyme Techniques, pubmed-meshheading:16968708-Lipid Metabolism, pubmed-meshheading:16968708-Lipolysis, pubmed-meshheading:16968708-Mass Spectrometry, pubmed-meshheading:16968708-Membrane Proteins, pubmed-meshheading:16968708-Mice, pubmed-meshheading:16968708-Mice, Knockout, pubmed-meshheading:16968708-Phospholipids, pubmed-meshheading:16968708-Pregnancy Proteins, pubmed-meshheading:16968708-RNA, Small Interfering, pubmed-meshheading:16968708-Triglycerides
pubmed:year
2006
pubmed:articleTitle
Functional compensation for adipose differentiation-related protein (ADFP) by Tip47 in an ADFP null embryonic cell line.
pubmed:affiliation
Geriatric Research, Education and Clinical Center, Baltimore Veterans Affairs Health Care Center, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA. csztalry@grecc.umaryland.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural