Linked Life Data

16968162

Source:http://linkedlifedata.com/resource/pubmed/id/16968162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-9-13
pubmed:abstractText
In the present work, we studied the differentiation capacity of mouse embryonic stem cells (mESCs) and mouse embryonic fibroblasts (MEFs) to differentiate into osteoblast-like cells in a 3-dimensional (3D) self-assembling peptide scaffold, a synthetic nanofiber biomaterial with potential applications in regenerative medicine. We demonstrated that 2D and 3D systems promoted differentiation of mESCs into cells with an osteoblast-like phenotype consisting of osteopontin and collagen I marker expression, as well as high alkaline phosphatase (ALP) activity and calcium phosphate deposits. In 3D cultures the frequency of appearance of embryonic stem cell-like colonies was substantially greater, suggesting that the 3D microenvironment promoted the generation of a stem cell-like niche that allows undifferentiated stem cell maintenance. On the other hand, after MEFs were cultured in the 3D system with their regular growth medium, but not in the 2D system, they expressed osteopontin, up-regulated metalloproteinase activities, and acquired a distinct phenotype consisting of small, elongated cells with remaining mitotic activity. Furthermore, only 3D MEF cultures underwent osteoblast differentiation after osteogenic induction, based on matrix mineralization, collagen I synthesis, ALP activity, and expression of the osteoblast transcription factor Runx2, suggesting that the 3D environment promotes differentiation of MEFs into osteoblast-like cells. We propose that the 3D system provides a unique microenvironment that promotes differentiation of mESCs and MEFs into osteoblast-like cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1076-3279
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2215-27
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Osteogenic differentiation of mouse embryonic stem cells and mouse embryonic fibroblasts in a three-dimensional self-assembling peptide scaffold.
pubmed:affiliation
Barcelona Bioengineering Center, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural