Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1990-9-20
|
| pubmed:abstractText |
CD40 mAb at subsaturating doses inhibit the growth of transformants of the M12 murine cell line expressing intact full length CD40 molecules (M12/CD40+ cells) but do not inhibit the growth of two M12 transformants expressing either a mutant CD40 cDNA missing most of the cytoplasmic tail (CD40/tailless) or a mutant cDNA with a substitution at residue 234 (CD40/234A, Ala for Thr). Using these transformants, we tested a panel of cytokines for the ability to mimic CD40 mAb. rIL-6 behaved like CD40 mAb and inhibited the growth of M12/CD40+ cells but not of CD40/tailless or CD40/234A mutants. The effect of IL-6 on M12/CD40+ cells not only required intact CD40 including threonine 234 but also was specific because IL-6 mAb blocked the inhibitory activity. The M12/CD40+ cells responsive to IL-6 expressed greater than 300,000 CD40 molecules/cells but, like M12/CD40-controls, expressed only small numbers (less than 50/cell) of high affinity IL-6R, indicating that CD40 is not a receptor for IL-6. Nevertheless, IL-6 utilizes intact CD40 efficiently when it signals these cells: treatment of M12/CD40+ cells with IL-6 induced increased phosphorylation of CD40. Conversely, triggering CD40 on M12/CD40+ cells leads to IL-6 production. Similar effects were evident in human CD40+ B cells: IL-6 increased the phosphorylation of CD40 in the IL-6-responsive cell line, CESS, and CD40 mAb induced IL-6 production in activated human B cells. Thus, CD40 may function to receive and regulate IL-6-dependent signals in B cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
145
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1400-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1696597-Animals,
pubmed-meshheading:1696597-Antibodies, Monoclonal,
pubmed-meshheading:1696597-Antigens, CD40,
pubmed-meshheading:1696597-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:1696597-B-Lymphocytes,
pubmed-meshheading:1696597-Cell Line,
pubmed-meshheading:1696597-Cloning, Molecular,
pubmed-meshheading:1696597-DNA Mutational Analysis,
pubmed-meshheading:1696597-Humans,
pubmed-meshheading:1696597-Interleukin-6,
pubmed-meshheading:1696597-Lymphocyte Activation,
pubmed-meshheading:1696597-Mice,
pubmed-meshheading:1696597-Phosphorylation,
pubmed-meshheading:1696597-Receptor Aggregation,
pubmed-meshheading:1696597-Receptors, Cell Surface,
pubmed-meshheading:1696597-Signal Transduction,
pubmed-meshheading:1696597-Structure-Activity Relationship,
pubmed-meshheading:1696597-Transfection
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Association between IL-6 and CD40 signaling. IL-6 induces phosphorylation of CD40 receptors.
|
| pubmed:affiliation |
Department of Microbiology, University of Washington, Seattle 98195.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|