Linked Life Data

1696316

Source:http://linkedlifedata.com/resource/pubmed/id/1696316

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-9-12
pubmed:abstractText
Ionic channels in a human monocyte cell line (U937) were studied with the inside-out patch-clamp technique. A Ca2(+)-activated K+ channel and three Cl- -selective channels were observed. The Ca2(+)-activated K+ channel had an inward-rectifying current-voltage relationship with slope conductance of 28 pS, and was not dependent on membrane potential. Among the three Cl- channels, an outward-rectifying 28-pS channel was most frequently observed. The permeability ratio (Cl-/Na+) was 4-5 and CH3SO4- was also permeant. The channel became less active with increasing polarizations in either direction, and was inactive beyond +/- 120 mV. The channel, observed as bursts, occasionally had rapid events within the bursts, suggesting the presence of another mode of kinetics. Diisothiocyanatostilbene-disulfonic acid (DIDS) blocked the channel reversibly in a dose-dependent manner. The second 328-pS Cl- channel had a linear current-voltage relationship and permeability ratio (Cl-/Na+) of 5-6. This channel became less active with increasing polarizations and inactive beyond +/- 50 mV. DIDS blocked the channel irreversibly. The channel had multiple subconductance states. The third 15-pS Cl- channel was least frequently observed and least voltage sensitive among the Cl- channels. Intracellular Ca2+ or pH affected none of the three Cl- channels. All three Cl- channels had a latent period before being observed, suggesting inhibitory factor(s) present in situ. Activation of the cells with interferon-gamma, interferon-alpha A or 12-O-tetradecanoylphorbol-13-acetate (TPA) caused no change in the properties of any of the channels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-61
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Chloride and potassium channels in U937 human monocytes.
pubmed:affiliation
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
pubmed:publicationType
Journal Article