Linked Life Data

1696270

Source:http://linkedlifedata.com/resource/pubmed/id/1696270

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-9-7
pubmed:abstractText
The conversion of latent transforming growth factor beta (LTGF-beta) to the active species, transforming growth factor beta (TGF-beta), has been characterized in heterotypic cultures of bovine aortic endothelial (BAE) cells and bovine smooth muscle cells (SMCs). The formation of TGF-beta in co-cultures of BAE cells and SMCs was documented by a specific radioreceptor competition assay, while medium from homotypic cultures of BAE cells or SMCs contained no active TGF-beta as determined by this assay. The concentration of TGF-beta in the conditioned medium of heterotypic co-cultures was estimated to be 400-1,200 pg/ml using the inhibition of BAE cell migration as an assay. Northern blotting of poly A+ RNA extracted from both homotypic and heterotypic cultures of BAE cells and SMCs revealed that BAE cells produced both TGF-beta 1 and TGF-beta 2, while SMCs produced primarily TGF-beta 1. No change in the expression of these two forms of TGF-beta was apparent after 24 h in heterotypic cultures. Time course studies on the appearance of TGF-beta indicated that most of the active TGF-beta was generated within the first 12 h after the establishment of co-cultures. The generation of TGF-beta in co-cultures stimulated the production of the protease inhibitor plasminogen activator inhibitor-1 (PAI-1). The inclusion of neutralizing antibodies to TGF-beta in the co-culture medium blocked the observed increase in PAI-1 levels. The increased expression of PAI-1 subsequent to TGF-beta formation blocked the activation of the protease required for conversion of LTGF-beta to TGF-beta as the inclusion of neutralizing antibodies to PAI-1 in the co-culture medium resulted in prolonged production of TGF-beta. This effect was lost upon removal of the PAI-1 antibodies. Thus, the activation of LTGF-beta appears to be a self-regulating system.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-183818, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2139036, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2472411, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2499579, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2519621, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2526131, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2629880, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2734305, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2820711, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2886992, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2890433, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-2967299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3007454, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3114269, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3162414, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3162913, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3163692, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3166463, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3417781, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3456347, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3458465, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3459460, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3461562, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3469200, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3475276, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3491081, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3493244, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3519251, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3654761, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-3930479, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6090474, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6093254, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6159641, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6299525, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6304530, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6602130, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6602340, http://linkedlifedata.com/resource/pubmed/commentcorrection/1696270-6607069
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
757-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Characterization of the activation of latent TGF-beta by co-cultures of endothelial cells and pericytes or smooth muscle cells: a self-regulating system.
pubmed:affiliation
Department of Cell Biology, New York University Medical Center, New York.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't