Linked Life Data

16955489

Source:http://linkedlifedata.com/resource/pubmed/id/16955489

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-10-2
pubmed:abstractText
Human intervertebral disc (IVD) degeneration often initiated from the human nucleus pulposus (hNP) with aging leading to IVD destruction and extracellular matrix (ECM) depletion. Previously, we have successfully employed transforming growth factor-beta1 (TGF-beta1) to promote chondrogenesis of mesenchymal progenitor cells (MPCs) and immortalized human mesenchymal stem cells. In this study, we examine the role of TGF-beta1 in platelet-rich plasma (PRP) on disc regeneration, including proliferation, redifferentiation, and the reconstitution of tissue-engineered NP. hNP cells were isolated from volunteers with different ages and cultured in the presence of PRP. We found that the most effective concentration for hNP proliferation was 1 ng/ml TGF-beta1 in PRP, which was further applied in the following experiments. hNP cell proliferation in all age groups were increased time-dependently by PRP and cell morphologies showed aggregation. The mRNA of Sox9, type II collagen, and aggrecan were all significantly upregulated by PRP through RT-PCR. Glycosaminoglycan (GAG) accumulation reached the highest value at day 7 and continued to day 9 culture. PRP promoted NP regeneration via the Smad pathway was also determined and highly activated p-Smad2/3 at 30 min and continuously sustained to 120 min. Immunostaining of type II collagen indicates that PRP participates in chondrogenesis of tissue-engineered NP with collagen scaffolds. We concluded that growth factors in PRP can effectively react as a growth factor cocktail to induce hNP proliferation and differentiation, and also promote tissue-engineered NP formation. These findings are the first to demonstrate that PRP might be a therapeutic candidate for prevention of disc degeneration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
(c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
209
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
744-54
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16955489-Adult, pubmed-meshheading:16955489-Aged, pubmed-meshheading:16955489-Animals, pubmed-meshheading:16955489-Apoptosis, pubmed-meshheading:16955489-Blood Platelets, pubmed-meshheading:16955489-Cell Proliferation, pubmed-meshheading:16955489-Chondrogenesis, pubmed-meshheading:16955489-Culture Media, Conditioned, pubmed-meshheading:16955489-Extracellular Matrix, pubmed-meshheading:16955489-Humans, pubmed-meshheading:16955489-Intervertebral Disc, pubmed-meshheading:16955489-Middle Aged, pubmed-meshheading:16955489-Plasma, pubmed-meshheading:16955489-Proteoglycans, pubmed-meshheading:16955489-RNA, Messenger, pubmed-meshheading:16955489-Regeneration, pubmed-meshheading:16955489-Smad2 Protein, pubmed-meshheading:16955489-Smad3 Protein, pubmed-meshheading:16955489-Tissue Engineering, pubmed-meshheading:16955489-Transforming Growth Factor beta1
pubmed:year
2006
pubmed:articleTitle
Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-beta1 in platelet-rich plasma.
pubmed:affiliation
Graduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan 110, ROC.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't