Linked Life Data

16952351

Source:http://linkedlifedata.com/resource/pubmed/id/16952351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2006-9-19
pubmed:abstractText
Glycyrrhizic acid is an herbal drug with a broad spectrum of antiviral activities and pharmacological effects and multiple sites of action. We investigated whether glycyrrhizic acid protects against glutamate-induced excitotoxicity and the underlying mechanisms. We found that glycyrrhizic acid protected against neurotoxicity in rat primary neuronal cultures and hippocampal slices by suppression of the glutamate-induced apoptosis. Glycyrrhizic acid conferred neuroprotective properties in a concentration-dependent manner, as determined by cell survival, apoptosis, and Ca(2+) influx. Glycyrrhizic acid selectively inhibited the Ca(2+) influx activated through N-methyl-D-aspartate (NMDA) receptor by glutamate, but not through membrane depolarization elicited by high K(+) induction. Glycyrrhizic acid treatment also diminished glutamate-induced DNA fragmentation and cleavage of poly (ADP-ribose) polymerase (PARP). Electrophoretic mobility shift assay (EMSA) indicated that glycyrrhizic acid inhibited the binding activity of nuclear factor kappaB (NF-kappaB) to its target elements. Western blot analysis of NF-kappaB inhibitor (IkappaBalpha) protein revealed that the inhibitory effect of glycyrrhizic acid on glutamate-induced activation of NF-kappaB activity was attributable to the inhibition of IkappaB kinase activity. Thus, the site of action of glycyrrhizic acid could be a downstream consequence of Ca(2+)entry through NMDA receptors and that NF-kappaB may be one downstream target in this process. These observations suggest that glycyrrhizic acid may be of therapeutic value for the prevention of cerebral damage elicited by the glutamate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
547
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-21
pubmed:meshHeading
pubmed-meshheading:16952351-2-Amino-5-phosphonovalerate, pubmed-meshheading:16952351-Animals, pubmed-meshheading:16952351-Apoptosis, pubmed-meshheading:16952351-Blotting, Western, pubmed-meshheading:16952351-Calcium, pubmed-meshheading:16952351-Caspase 3, pubmed-meshheading:16952351-Cell Survival, pubmed-meshheading:16952351-Cells, Cultured, pubmed-meshheading:16952351-DNA Fragmentation, pubmed-meshheading:16952351-Dose-Response Relationship, Drug, pubmed-meshheading:16952351-Electrophoretic Mobility Shift Assay, pubmed-meshheading:16952351-Female, pubmed-meshheading:16952351-Glutamic Acid, pubmed-meshheading:16952351-Glycyrrhizic Acid, pubmed-meshheading:16952351-Membrane Potentials, pubmed-meshheading:16952351-NF-kappa B, pubmed-meshheading:16952351-Neurons, pubmed-meshheading:16952351-Neuroprotective Agents, pubmed-meshheading:16952351-Poly(ADP-ribose) Polymerases, pubmed-meshheading:16952351-Protein Binding, pubmed-meshheading:16952351-Rats, pubmed-meshheading:16952351-Rats, Sprague-Dawley, pubmed-meshheading:16952351-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:16952351-Tetrodotoxin
pubmed:year
2006
pubmed:articleTitle
Inhibition of nuclear factor kappaB is associated with neuroprotective effects of glycyrrhizic acid on glutamate-induced excitotoxicity in primary neurons.
pubmed:affiliation
Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan, ROC.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't