Linked Life Data

16952011

Source:http://linkedlifedata.com/resource/pubmed/id/16952011

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-10-2
pubmed:abstractText
In this rapid communication we describe a new approach to protein detection with chemiluminescence. By combining common practices in protein detection with chemiluminescence, microwave technology, and metal-enhanced chemiluminescence, we show that we can use low power microwaves to substantially increase enzymatic chemiluminescent reaction rates on metal substrates. As a result, we have found that we can in essence trigger chemiluminescence with low power microwave (Mw) pulses and ultimately, perform on-demand protein detection assays. Using microwave triggered metal-enhanced chemiluminescence (MT-MEC), we not only improve the sensitivity of immunoassays with enhanced signal-to-noise ratios, but we also show that we can accurately quantify protein concentrations by integrating the photon flux for discrete time intervals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1053-0509
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
641-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Microwave-triggered metal-enhanced chemiluminescence (MT-MEC): application to ultra-fast and ultra-sensitive clinical assays.
pubmed:affiliation
Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, Institute of Fluorescence, University of Maryland Biotechnology Institute, 725 West Lombard St., Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural