Source:http://linkedlifedata.com/resource/pubmed/id/16951344
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2006-9-4
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pubmed:abstractText |
Intracellular differentiation events that determine which cells develop into memory CD8 T cells are currently incompletely understood. Methyl-CpG-binding domain protein 2 (MBD2) is a transcriptional repressor that binds to methylated DNA and mediates the biological consequences of epigenetic gene methylation. The role of MBD2 during the differentiation of naive CD8 T cells into effector and memory cells was determined following acute infection of MBD2-deficient mice with lymphocytic choriomeningitis virus. Despite rapid viral clearance and an efficient primary effector CD8 T cell response, reduced numbers of Ag-specific memory CD8 T cells were observed. Importantly, the appearance of precursor memory cells (IL-7Ralphahigh) was delayed. The remaining MBD2(-/-) memory cells were not fully protective during rechallenge, and memory cell characteristics were altered with regard to surface markers (IL-7Ralpha, KLRG-1, CD27, and others) and cytokine production. The defect was CD8 T cell intrinsic, because memory cell development was also delayed when MBD2(-/-) CD8 T cells were adoptively transferred into SCID mice. These data demonstrate that MBD2 is a previously unrecognized intracellular factor required for the efficient generation of protective memory CD8 T cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3821-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16951344-Animals,
pubmed-meshheading:16951344-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16951344-Cell Differentiation,
pubmed-meshheading:16951344-Cells, Cultured,
pubmed-meshheading:16951344-CpG Islands,
pubmed-meshheading:16951344-DNA Methylation,
pubmed-meshheading:16951344-DNA-Binding Proteins,
pubmed-meshheading:16951344-Female,
pubmed-meshheading:16951344-Immunologic Memory,
pubmed-meshheading:16951344-Male,
pubmed-meshheading:16951344-Mice,
pubmed-meshheading:16951344-Mice, Inbred BALB C,
pubmed-meshheading:16951344-Mice, Inbred C57BL,
pubmed-meshheading:16951344-Mice, Knockout,
pubmed-meshheading:16951344-Mice, SCID,
pubmed-meshheading:16951344-Protein Binding,
pubmed-meshheading:16951344-Protein Structure, Tertiary,
pubmed-meshheading:16951344-Stem Cells
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pubmed:year |
2006
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pubmed:articleTitle |
Impaired memory CD8 T cell development in the absence of methyl-CpG-binding domain protein 2.
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pubmed:affiliation |
Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA. ekersh@emory.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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