Linked Life Data

16951047

Source:http://linkedlifedata.com/resource/pubmed/id/16951047

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:16951047rdf:typepubmed:Citationlld:pubmed
pubmed-article:16951047lifeskim:mentionsumls-concept:C0007634lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1332717lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1706438lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0018787lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0450127lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0085358lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0070410lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0038952lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1512840lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1413244lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C2698600lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0392756lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0033268lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1879547lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C1515895lld:lifeskim
pubmed-article:16951047lifeskim:mentionsumls-concept:C0205228lld:lifeskim
pubmed-article:16951047pubmed:issue1lld:pubmed
pubmed-article:16951047pubmed:dateCreated2007-1-10lld:pubmed
pubmed-article:16951047pubmed:abstractTextPerforin is a cytolytic mediator produced by cytotoxic T cells (CD8(+) cells) and natural killer cells. We previously reported that ex vivo IL-10 gene therapy induced apoptosis of allogenic infiltrative CD8(+) cells and significantly prolonged cardiac allograft survival. To further test the hypothesis that localized IL-10 overexpression in cardiac allografts may also effect the alloreactive CD8(+) T cell function by downregulating its perforin production, we used a rabbit functional heterotopic allograft heart transplant model. Human recombinant IL-10 gene complexed with liposome was intracoronary delivered into the cardiac allografts ex vivo. The percentage of apoptotic infiltrative CD8(+) cells in cardiac allografts was increased 6-fold in the gene therapy group vs. the control group, whereas the percentage of perforin-positive CD8(+) cells was decreased 2.9-fold (P < 0.01). Perforin expression level in the allograft myocardium of the gene therapy group was deceased 3.2-fold (P < 0.01). The amount of infiltrative perforin-positive CD8(+) cells and perforin expression level were inversely correlated with IL-10 transgene and protein expression level in the myocardium of cardiac allografts (P < 0.01), the percentage of apoptotic cardiac myocytes (P < 0.01), and the peak left ventricular systolic pressure of cardiac allografts (P < 0.01) but significantly correlated with the infiltrative T cell cytotoxicity (P < 0.01) and allograft rejection score (P < 0.01). These results suggest that localized IL-10 gene therapy prolongs cardiac allograft survival, at least in part, through downregulation of perforin production by activated allogenic CD8(+) T cells. Reduction of cytolytic function of cytotoxic effector cells prevents the apoptosis of cardiac myocytes.lld:pubmed
pubmed-article:16951047pubmed:languageenglld:pubmed
pubmed-article:16951047pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16951047pubmed:citationSubsetIMlld:pubmed
pubmed-article:16951047pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16951047pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16951047pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16951047pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16951047pubmed:statusMEDLINElld:pubmed
pubmed-article:16951047pubmed:monthJanlld:pubmed
pubmed-article:16951047pubmed:issn0363-6135lld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:LaksHillelHlld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:MOONM WMWlld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:OshimaKiyohir...lld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:CuiGuanggenGlld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:TungThomasTlld:pubmed
pubmed-article:16951047pubmed:authorpubmed-author:OkotieOnisuru...lld:pubmed
pubmed-article:16951047pubmed:issnTypePrintlld:pubmed
pubmed-article:16951047pubmed:volume292lld:pubmed
pubmed-article:16951047pubmed:ownerNLMlld:pubmed
pubmed-article:16951047pubmed:authorsCompleteYlld:pubmed
pubmed-article:16951047pubmed:paginationH277-84lld:pubmed
pubmed-article:16951047pubmed:dateRevised2007-11-15lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:meshHeadingpubmed-meshheading:16951047...lld:pubmed
pubmed-article:16951047pubmed:year2007lld:pubmed
pubmed-article:16951047pubmed:articleTitleExogenous IL-10 overexpression reduces perforin production by activated allogenic CD8+ cells and prolongs cardiac allograft survival.lld:pubmed
pubmed-article:16951047pubmed:affiliationDivision of Cardiothoracic Surgery, Dept. of Surgery, UCLA Medical Center, David Geffen School of Medicine in UCLA, 10833 Le Conte Ave., 47-123 CHS, Los Angeles, CA 90095-1679, USA.lld:pubmed
pubmed-article:16951047pubmed:publicationTypeJournal Articlelld:pubmed