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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1990-8-15
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pubmed:abstractText |
The human interleukin 3 (IL-3) promoter is comprised of several cis-acting DNA sequences that modulate T-cell expression of IL-3. These are located within 315 nucleotides upstream of the mRNA start site. Transient expression of reporter genes linked to serially deleted sequences of the IL-3 promoter has allowed mapping of two activator sequences and an interposed repressor sequence. The proximal regulatory region is specific to IL-3 and prerequisite for efficient transcription. Its effect is enhanced by a second, more distal activating sequence consisting of an AP-1 binding site. Between the two activators lies a transcriptional silencer, which is a potent repressor in the absence of the AP-1 site. DNA-nuclear protein binding experiments demonstrate specific complex formation within each of these functional regions. Thus, both positive and negative regulatory elements appear to control expression of the human IL-3 gene in activated T cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2437014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2437015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2467208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2572326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2595372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2645952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2833332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-2875459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3034432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3034976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3127463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3141060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3257571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-3296190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-4501200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-6313211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-6324170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1695008-6828386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5046-50
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1695008-Base Sequence,
pubmed-meshheading:1695008-Cell Line,
pubmed-meshheading:1695008-Deoxyribonuclease I,
pubmed-meshheading:1695008-Gene Expression,
pubmed-meshheading:1695008-Gene Expression Regulation,
pubmed-meshheading:1695008-Humans,
pubmed-meshheading:1695008-Interleukin-3,
pubmed-meshheading:1695008-Lymphocyte Activation,
pubmed-meshheading:1695008-Molecular Sequence Data,
pubmed-meshheading:1695008-Oligonucleotide Probes,
pubmed-meshheading:1695008-Promoter Regions, Genetic,
pubmed-meshheading:1695008-RNA,
pubmed-meshheading:1695008-Ribonucleases,
pubmed-meshheading:1695008-T-Lymphocytes,
pubmed-meshheading:1695008-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Positive and negative elements regulate human interleukin 3 expression.
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pubmed:affiliation |
Division of Hematology Oncology, Children's Hospital, Boston MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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