Source:http://linkedlifedata.com/resource/pubmed/id/16946264
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 9
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| pubmed:dateCreated |
2006-9-1
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| pubmed:abstractText |
Nine mercury-resistance plasmids isolated from river epilithon were assessed for their ability to retrotransfer the non-conjugative IncQ plasmid, R300B, derivatives of which have commercial uses that may result in accidental or deliberate release into the environment. Retrotransfer frequencies ranging from 2.1 x 10(-4) to 1.75 x 10(-5) were obtained for five of the nine plasmids--the remaining plasmids showed low or undetectable retrotransfer ability. The majority of the retrotransfer-proficient plasmids could not be classified by the tests used. Classical incompatibility testing with RP4 identified pQKH6, pQKH54 and pQM719 as IncP-1. Hybridization to replicon probes confirmed this for pQKH6 and pQM719 and added pQKH33. PCR with primers designed to amplify trfA and korA regions of IncP-1 plasmids did not identify any other plasmids. Plasmids pQKH6 and pQM719 but not pQKH54 produced similar SphI restriction profiles to the IncP-1beta subgroup. The complete nucleotide sequence of pQKH54 was determined, revealing it to have a complete IncP-1 backbone but belonging to a new distinct subgroup which was designated IncP-1gamma. The results emphasize the ubiquity and diversity of IncP-1 plasmids in the environment but demonstrate that plasmids of as yet unknown groups are also able to retrotransfer IncQ plasmids efficiently.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Mercury,
http://linkedlifedata.com/resource/pubmed/chemical/Retroelements
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
152
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2689-701
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| pubmed:dateRevised |
2007-8-13
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| pubmed:meshHeading |
pubmed-meshheading:16946264-Base Composition,
pubmed-meshheading:16946264-Base Sequence,
pubmed-meshheading:16946264-Conjugation, Genetic,
pubmed-meshheading:16946264-DNA, Bacterial,
pubmed-meshheading:16946264-DNA Primers,
pubmed-meshheading:16946264-DNA Probes,
pubmed-meshheading:16946264-DNA Restriction Enzymes,
pubmed-meshheading:16946264-Drug Resistance, Bacterial,
pubmed-meshheading:16946264-Environment,
pubmed-meshheading:16946264-Fresh Water,
pubmed-meshheading:16946264-Mercury,
pubmed-meshheading:16946264-Models, Genetic,
pubmed-meshheading:16946264-Molecular Sequence Data,
pubmed-meshheading:16946264-Nucleic Acid Amplification Techniques,
pubmed-meshheading:16946264-Nucleic Acid Hybridization,
pubmed-meshheading:16946264-Phylogeny,
pubmed-meshheading:16946264-Plasmids,
pubmed-meshheading:16946264-Polymerase Chain Reaction,
pubmed-meshheading:16946264-Replication Origin,
pubmed-meshheading:16946264-Replicon,
pubmed-meshheading:16946264-Restriction Mapping,
pubmed-meshheading:16946264-Retroelements,
pubmed-meshheading:16946264-Sequence Analysis, DNA
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| pubmed:year |
2006
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| pubmed:articleTitle |
Plasmids from freshwater environments capable of IncQ retrotransfer are diverse and include pQKH54, a new IncP-1 subgroup archetype.
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| pubmed:affiliation |
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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