| pubmed-article:16946001 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C0162371 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C0882849 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C2348480 | lld:lifeskim |
| pubmed-article:16946001 | lifeskim:mentions | umls-concept:C1516240 | lld:lifeskim |
| pubmed-article:16946001 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:16946001 | pubmed:dateCreated | 2006-9-1 | lld:pubmed |
| pubmed-article:16946001 | pubmed:abstractText | We examined whether ex vivo expansion of umbilical cord blood progenitor cells affected their capacity to generate immune cells such as T lymphocytes (TLs) and dendritic cells (DCs). The capacity to generate TLs from cord blood CD34(+) cells expanded for 14 days (d14) was compared with that of nonexpanded CD34(+) cells (d0) using fetal thymus organ cultures or transfer into nonobese diabetic/severe combined immunodeficient mice. The cell preparations yielded comparable percentages of immature (CD4(+)CD8(-), CD4(+)CD8(+)) TLs and functional mature (CD3(+)CD4(+), CD3(+)CD8(+)) TLs with an analogous TCR (T-cell receptor)-Vbeta repertoire pattern. As regards DCs, d0 and d14 CD34(+) cells also yielded similar percentages of CD1a(+) DCs with the same expression levels of HLA-DR, costimulatory and adhesion molecules, and chemokine receptors. DCs derived from either d14 or d0 CD34(+) stimulated allogeneic TLs to the same extent, and the cytokine pattern production of these allogeneic TLs was similar with no shift toward a predominant Th1 or Th2 response. Even though the intrinsic capacity of d14 CD34(+) cells to generate DCs was 13-fold lower than that of d0 CD34(+) cells, this reduction was offset by the prior amplification of the CD34(+) cells, resulting in the overall production of 15-fold more DCs. These data indicate that ex vivo expansion of CD34(+) cells does not impair T lymphopoiesis nor DC differentiation capacity. | lld:pubmed |
| pubmed-article:16946001 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16946001 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16946001 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16946001 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16946001 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16946001 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16946001 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16946001 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16946001 | pubmed:issn | 1066-5099 | lld:pubmed |
| pubmed-article:16946001 | pubmed:author | pubmed-author:RosenzwajgMic... | lld:pubmed |
| pubmed-article:16946001 | pubmed:author | pubmed-author:KobariLadanL | lld:pubmed |
| pubmed-article:16946001 | pubmed:author | pubmed-author:DouayLucL | lld:pubmed |
| pubmed-article:16946001 | pubmed:author | pubmed-author:GiarratanaMar... | lld:pubmed |
| pubmed-article:16946001 | pubmed:author | pubmed-author:GluckmanJean... | lld:pubmed |
| pubmed-article:16946001 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16946001 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:16946001 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16946001 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16946001 | pubmed:pagination | 2150-7 | lld:pubmed |
| pubmed-article:16946001 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
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| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:meshHeading | pubmed-meshheading:16946001... | lld:pubmed |
| pubmed-article:16946001 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16946001 | pubmed:articleTitle | Ex vivo expansion does not alter the capacity of umbilical cord blood CD34+ cells to generate functional T lymphocytes and dendritic cells. | lld:pubmed |
| pubmed-article:16946001 | pubmed:affiliation | Laboratoire d'Hématologie, Unité de Formation et de Recherche EA1638, Université Pierre et Marie Curie, CHU Saint Antoine, Paris, France. | lld:pubmed |
| pubmed-article:16946001 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16946001 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16946001 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16946001 | lld:pubmed |
