Linked Life Data

16944345

Source:http://linkedlifedata.com/resource/pubmed/id/16944345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-5-7
pubmed:databankReference
pubmed:abstractText
The bacterial community in soil was screened by using various molecular approaches for bacterial populations that were activated upon addition of different supplements. Plasmodiophora brassicae spores, chitin, sodium acetate, and cabbage plants were added to activate specific bacterial populations as an aid in screening for novel antagonists to plant pathogens. DNA from growing bacteria was specifically extracted from the soil by bromodeoxyuridine immunocapture. The captured DNA was fingerprinted by terminal restriction fragment length polymorphism (T-RFLP). The composition of the dominant bacterial community was also analyzed directly by T-RFLP and by denaturing gradient gel electrophoresis (DGGE). After chitin addition to the soil, some bacterial populations increased dramatically and became dominant both in the total and in the actively growing community. Some of the emerging bands on DGGE gels from chitin-amended soil were sequenced and found to be similar to known chitin-degrading genera such as Oerskovia, Kitasatospora, and Streptomyces species. Some of these sequences could be matched to specific terminal restriction fragments on the T-RFLP output. After addition of Plasmodiophora spores, an increase in specific Pseudomonads could be observed with Pseudomonas-specific primers for DGGE. These results demonstrate the utility of microbiomics, or a combination of molecular approaches, for investigating the composition of complex microbial communities in soil.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0095-3628
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-413
pubmed:meshHeading
pubmed-meshheading:16944345-Bacteria, pubmed-meshheading:16944345-Base Sequence, pubmed-meshheading:16944345-Biodiversity, pubmed-meshheading:16944345-Brassica, pubmed-meshheading:16944345-Bromodeoxyuridine, pubmed-meshheading:16944345-Chitin, pubmed-meshheading:16944345-Cluster Analysis, pubmed-meshheading:16944345-Ecosystem, pubmed-meshheading:16944345-Electrophoresis, Agar Gel, pubmed-meshheading:16944345-Fungi, pubmed-meshheading:16944345-Molecular Sequence Data, pubmed-meshheading:16944345-Plant Diseases, pubmed-meshheading:16944345-Plant Roots, pubmed-meshheading:16944345-Polymorphism, Restriction Fragment Length, pubmed-meshheading:16944345-Pseudomonas, pubmed-meshheading:16944345-RNA, Ribosomal, 16S, pubmed-meshheading:16944345-Sodium Acetate, pubmed-meshheading:16944345-Soil Microbiology, pubmed-meshheading:16944345-Spores, Fungal
pubmed:year
2007
pubmed:articleTitle
Community structure of actively growing bacterial populations in plant pathogen suppressive soil.
pubmed:affiliation
Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, SE-750 07, Uppsala, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't