Linked Life Data

16943582

Source:http://linkedlifedata.com/resource/pubmed/id/16943582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-8-31
pubmed:abstractText
Very few of the genes that are important in pituitary tumor initiation, progression, and metastasis have been identified to date. To identify potential genes that may be important in pituitary tumor progression and carcinoma development, we used Affymetrix GeneChip HGU-133A-oligonucleotide arrays, which contain more than 15,000 characterized genes from the human genome to study gene expression in an ACTH pituitary carcinoma metastatic to the liver and four pituitary adenomas. Reverse-transcriptase real-time quantitative- PCR (RT-qPCR) was then used to analyze 4 nonneoplastic pituitaries, 19 adenomas, and the ACTH carcinoma. A larger series of pituitary adenomas and carcinomas were also analyzed for protein expression using tissue microarrays (TMA) (n = 233) and by Western blotting (n = 18). There were 4298 genes that were differentially expressed among the adenomas compared to the carcinoma, with 2057 genes overexpressed and 2241 genes underexpressed in the adenomas. The beta-galactoside binding protein galactin-3 was underexpressed in some adenomas compared to the carcinomas. Prolactin (PRL) and ACTH tumors had the highest levels of expression of galectin-3. The human achaetescute homolog-1 ASCL1 (hASH-1) gene was also underexpressed in some adenomas compared to the carcinoma. Prolactin and ACTH tumors had the highest levels of expression of hASH-1. ID2, which has an important role in cell development and tumorigenesis, was underexpressed in some adenomas compared to the carcinomas. Transducin-like enhancer of split four/ Groucho (TLE-4) was over-expressed in adenomas compared to the ACTH carcinoma. The differential expression of these genes was validated by RT-qPCR, by immunohistochemistry using TMA and by Western blotting. These results indicate that the LGALS3, hASH1, ID2, and TLE-4 genes may have important roles in the development of pituitary carcinomas.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1355-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435-44
pubmed:dateRevised
2010-6-24
pubmed:meshHeading
pubmed-meshheading:16943582-ACTH-Secreting Pituitary Adenoma, pubmed-meshheading:16943582-Adenoma, pubmed-meshheading:16943582-Blotting, Western, pubmed-meshheading:16943582-Carcinoma, pubmed-meshheading:16943582-DNA-Binding Proteins, pubmed-meshheading:16943582-Follicle Stimulating Hormone, pubmed-meshheading:16943582-GRB2 Adaptor Protein, pubmed-meshheading:16943582-Gene Expression Profiling, pubmed-meshheading:16943582-Growth Hormone-Secreting Pituitary Adenoma, pubmed-meshheading:16943582-Humans, pubmed-meshheading:16943582-Immunohistochemistry, pubmed-meshheading:16943582-Inhibitor of Differentiation Protein 2, pubmed-meshheading:16943582-Luteinizing Hormone, pubmed-meshheading:16943582-Nuclear Proteins, pubmed-meshheading:16943582-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16943582-Pituitary Neoplasms, pubmed-meshheading:16943582-Prolactinoma, pubmed-meshheading:16943582-Repressor Proteins, pubmed-meshheading:16943582-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2006
pubmed:articleTitle
Patterns of gene expression in pituitary carcinomas and adenomas analyzed by high-density oligonucleotide arrays, reverse transcriptase-quantitative PCR, and protein expression.
pubmed:affiliation
Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 200 First Street, SW, Rochester, MN 55905, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural, Validation Studies