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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2006-9-6
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pubmed:abstractText |
Argonaute proteins are the core components of effector complexes that facilitate RNA interference (RNAi). Small interfering RNAs (siRNAs) targeted to promoter regions mediate transcriptional gene silencing (TGS) in human cells through heterochromatin formation. RNAi effector complexes have yet to be implicated in the mechanism of mammalian TGS. Here we describe the role of the human Argonaute-1 homolog (AGO1) in directing TGS at the promoters for human immunodeficiency virus-1 coreceptor CCR5 and tumor suppressor RASSF1A. AGO1 associates with RNA polymerase II (RNAPII) and is required for histone H3 Lys9 dimethylation and TGS. AGO1, TAR RNA-binding protein-2 (7TRBP2) and Polycomb protein EZH2 colocalize to the siRNA-targeted RASSF1A promoter, implicating Polycomb silencing in the mechanism of mammalian TGS. These results establish a connection between RNAi components AGO1 and TRBP2, RNAPII transcription and Polycomb-regulated control of gene expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EIF2C1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/EZH2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/RASSF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR5,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/polycomb group proteins,
http://linkedlifedata.com/resource/pubmed/chemical/trans-activation responsive...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1545-9993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
793-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16936726-Argonaute Proteins,
pubmed-meshheading:16936726-Cells, Cultured,
pubmed-meshheading:16936726-DNA Methylation,
pubmed-meshheading:16936726-DNA-Binding Proteins,
pubmed-meshheading:16936726-Eukaryotic Initiation Factors,
pubmed-meshheading:16936726-HeLa Cells,
pubmed-meshheading:16936726-Histones,
pubmed-meshheading:16936726-Humans,
pubmed-meshheading:16936726-Phosphorylation,
pubmed-meshheading:16936726-Promoter Regions, Genetic,
pubmed-meshheading:16936726-RNA, Small Interfering,
pubmed-meshheading:16936726-RNA Interference,
pubmed-meshheading:16936726-RNA Polymerase II,
pubmed-meshheading:16936726-RNA-Binding Proteins,
pubmed-meshheading:16936726-Receptors, CCR5,
pubmed-meshheading:16936726-Repressor Proteins,
pubmed-meshheading:16936726-Transcription Factors,
pubmed-meshheading:16936726-Tumor Suppressor Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells.
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pubmed:affiliation |
Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, 1450 E. Duarte Road, Duarte, California 91010, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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