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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
45
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pubmed:dateCreated |
2006-11-6
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pubmed:databankReference |
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pubmed:abstractText |
A novel 2986-base transcript encoded by the antisense strand of the HRES-1 human endogenous retrovirus was isolated from peripheral blood lymphocytes. This transcript codes for a 218-amino acid protein, termed HRES-1/Rab4, based on homology to the Rab4 family of small GTPases. Antibody 13407 raised against recombinant HRES-1/Rab4 detected a native protein of identical molecular weight in human T cells. HRES-1 nucleotides 2151-1606, located upstream of HRES-1/Rab4 exon 1, have promoter activity when oriented in the direction of HRES-1/Rab4 transcription. The human immunodeficiency virus, type 1 (HIV-1), tat gene stimulates transcriptional activity of the HRES-1/Rab4 promoter via trans-activation of the HRES-1 long terminal repeat. Transfection of HIV-1 tat into HeLa cells or infection of H9 and Jurkat cells by HIV-1 increased HRES-1/Rab4 protein levels. Overexpression of HRES-1/Rab4 in Jurkat cells abrogated HIV infection, gag p24 production, and apoptosis, whereas dominant-negative HRES-1/Rab4(S27N) had the opposite effects. HRES-1/Rab4 inhibited surface expression of CD4 and targeted it for lysosomal degradation. HRES-1/Rab4(S27N) enhanced surface expression, recycling, and total cellular CD4 content. Infection by HIV elicited a coordinate down-regulation of CD4 and up-regulation of HRES-1/Rab4 in PBL. Moreover, overexpression of HRES-1/Rab4 reduced CD4 expression on peripheral blood CD4+ T cells. Stimulation by HIV-1 of HRES-1/Rab4 expression and its regulation of CD4 recycling reveal novel coordinate interactions between an infectious retrovirus and the human genome.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/CD71 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rab4 GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BankiKatalinK,
pubmed-author:FernandezDavidD,
pubmed-author:GergelyPeterPJr,
pubmed-author:GrossmanCraig ECE,
pubmed-author:KonczAgnesA,
pubmed-author:MosserDick DDD,
pubmed-author:NagyGyorgyG,
pubmed-author:NilandBrianB,
pubmed-author:PerlAndrasA,
pubmed-author:SpivaFloyd PFPJr,
pubmed-author:StancatoChristinaC,
pubmed-author:TelaricoTiffanyT,
pubmed-author:WardJeffreyJ
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
34574-91
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16935861-Antigens, CD,
pubmed-meshheading:16935861-Antigens, CD4,
pubmed-meshheading:16935861-Apoptosis,
pubmed-meshheading:16935861-Base Sequence,
pubmed-meshheading:16935861-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16935861-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:16935861-Dependovirus,
pubmed-meshheading:16935861-Disease Susceptibility,
pubmed-meshheading:16935861-Exons,
pubmed-meshheading:16935861-Flow Cytometry,
pubmed-meshheading:16935861-Gene Expression Regulation, Viral,
pubmed-meshheading:16935861-Gene Products, tat,
pubmed-meshheading:16935861-Genes, Dominant,
pubmed-meshheading:16935861-HIV Core Protein p24,
pubmed-meshheading:16935861-HIV Infections,
pubmed-meshheading:16935861-HIV Long Terminal Repeat,
pubmed-meshheading:16935861-HIV-1,
pubmed-meshheading:16935861-HeLa Cells,
pubmed-meshheading:16935861-Humans,
pubmed-meshheading:16935861-Introns,
pubmed-meshheading:16935861-Jurkat Cells,
pubmed-meshheading:16935861-Lysosomes,
pubmed-meshheading:16935861-Molecular Sequence Data,
pubmed-meshheading:16935861-Mutagenesis, Site-Directed,
pubmed-meshheading:16935861-Mutation,
pubmed-meshheading:16935861-Receptors, Transferrin,
pubmed-meshheading:16935861-Recombinant Fusion Proteins,
pubmed-meshheading:16935861-Sequence Homology, Nucleic Acid,
pubmed-meshheading:16935861-Transfection,
pubmed-meshheading:16935861-rab4 GTP-Binding Proteins,
pubmed-meshheading:16935861-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2006
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pubmed:articleTitle |
Regulation of CD4 expression via recycling by HRES-1/RAB4 controls susceptibility to HIV infection.
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pubmed:affiliation |
Department of Medicine, State University of New York, College of Medicine, Syracuse, New York 13210, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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