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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2006-11-20
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pubmed:abstractText |
Nociceptin/orphanin FQ (NOP/OFQ) is the endogenous ligand for the NOP receptor and is processed from a precursor protein in the family of opioid peptides. Prepronociceptin (ppN/OFQ) mRNA has been shown to be upregulated by an increase in cAMP, a treatment that leads to differentiation of NS20Y neuroblastoma cells. Although a large increase in endogenous ppN/OFQ mRNA upon cAMP stimulation can be shown in cellular systems, a similar increase cannot be expressed in pGL3 luciferase vector containing 1.3 kb proximal promoter, suggesting that a larger portion of the sequence or a different chromatin structure is necessary for a fully functional promoter. The induction of ppN/OFQ mRNA by cAMP appears to be mediated by a cAMP-response element. Chromatin immunoprecipitation (ChIP) assays show that CREB is recruited to the promoter region upon treatment of NS20Y cells with dibutyryl cAMP. In addition, the production of ppN/OFQ mRNA is regulated by histone acetylation, also through CREB, as the histone deacetylase (HDAC) inhibitor trichostatin A increases both CREB binding to the promoter and ppN/OFQ mRNA expression. In rat progenitor and mouse neuroblastoma cell lines, agents that increase ppN/OFQ mRNA expression also induce neurite outgrowth, suggesting a close relationship between ppN/OFQ and cellular differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxamic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-(4-bromocinnamylamino)ethyl)-5-...,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Plicamycin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/mithramycin A,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin,
http://linkedlifedata.com/resource/pubmed/chemical/prepronociceptin,
http://linkedlifedata.com/resource/pubmed/chemical/trichostatin A
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
384
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-36
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16935438-Animals,
pubmed-meshheading:16935438-Base Sequence,
pubmed-meshheading:16935438-Bucladesine,
pubmed-meshheading:16935438-Cell Differentiation,
pubmed-meshheading:16935438-Cell Line,
pubmed-meshheading:16935438-Chromatin Immunoprecipitation,
pubmed-meshheading:16935438-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:16935438-Fetus,
pubmed-meshheading:16935438-Gene Expression Regulation, Developmental,
pubmed-meshheading:16935438-Hippocampus,
pubmed-meshheading:16935438-Humans,
pubmed-meshheading:16935438-Hydroxamic Acids,
pubmed-meshheading:16935438-Isoquinolines,
pubmed-meshheading:16935438-Mice,
pubmed-meshheading:16935438-Molecular Sequence Data,
pubmed-meshheading:16935438-Neurons,
pubmed-meshheading:16935438-Opioid Peptides,
pubmed-meshheading:16935438-Plicamycin,
pubmed-meshheading:16935438-Promoter Regions, Genetic,
pubmed-meshheading:16935438-Protein Precursors,
pubmed-meshheading:16935438-Rats,
pubmed-meshheading:16935438-Rats, Sprague-Dawley,
pubmed-meshheading:16935438-Receptors, Opioid,
pubmed-meshheading:16935438-Sequence Alignment,
pubmed-meshheading:16935438-Sulfonamides,
pubmed-meshheading:16935438-Transcription, Genetic,
pubmed-meshheading:16935438-Transfection
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pubmed:year |
2006
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pubmed:articleTitle |
Regulation of the prepronociceptin gene and its effect on neuronal differentiation.
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pubmed:affiliation |
SRI International, Biosciences Division, 333 Ravenswood Ave, LA214A, Menlo Park, CA 94025, USA. naunihal.zaveri@sri.com
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, N.I.H., Extramural
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