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rdf:type |
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lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0031751,
umls-concept:C0055367,
umls-concept:C0086140,
umls-concept:C0205349,
umls-concept:C0560175,
umls-concept:C0681828,
umls-concept:C1145667,
umls-concept:C1167622,
umls-concept:C1706209,
umls-concept:C2349209,
umls-concept:C2825311
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pubmed:issue |
11
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pubmed:dateCreated |
1990-7-12
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pubmed:abstractText |
A monoclonal antibody-dextran-Sn(IV) chlorin e6 immunoconjugate was prepared by a technique involving the site-specific covalent modification of the monoclonal antibody oligosaccharide moiety. Dextran carriers were synthesized with a single chain-terminal hydrazide group, which was used as the coupling point between the carrier and the monoclonal antibody carbohydrate. Selective in vitro photolysis of SK-MEL-2 human malignant melanoma cells was accomplished using several conjugates prepared from anti-melanoma 2.1 (chromophore:antibody molar ratios, 6.8 and 11.2). Phototoxicity, as measured by clonogenic assay, was dependent on the delivered dose of 634-nm light and was observed only for conjugates that bound SK-MEL-2 cells. As judged by competitive inhibition radioimmunoassay, conjugates prepared in this fashion showed excellent retention of antigen binding activity relative to the unmodified antibody.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-2453476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-2877461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-2980947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3010344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3035026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3149991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3406124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3458222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3536124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-365720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-3905969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6185591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6234364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6256319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6341465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6346011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6366145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6836304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6852982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-6968914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-7042536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1693435-7384803
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4217-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1693435-Antibodies, Monoclonal,
pubmed-meshheading:1693435-Antibodies, Neoplasm,
pubmed-meshheading:1693435-Chemical Phenomena,
pubmed-meshheading:1693435-Chemistry,
pubmed-meshheading:1693435-Dextrans,
pubmed-meshheading:1693435-Humans,
pubmed-meshheading:1693435-Immunotoxins,
pubmed-meshheading:1693435-Photochemistry,
pubmed-meshheading:1693435-Porphyrins,
pubmed-meshheading:1693435-Tin,
pubmed-meshheading:1693435-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Antibody-targeted photolysis: in vitro studies with Sn(IV) chlorin e6 covalently bound to monoclonal antibodies using a modified dextran carrier.
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pubmed:affiliation |
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ 08855-0909.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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