Linked Life Data

16933606

Source:http://linkedlifedata.com/resource/pubmed/id/16933606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-8-28
pubmed:abstractText
A novel bacterial decolorization enzyme for triphenylmethane dyes from Aeromonas hydrophila strain DN322 was purified, named TpmD. The purified enzyme catalyzes the decolorization of several triphenylmethane dyes, i.e., crystal violet, basic fuchsin, brilliant green and malachite green. The enzyme was identified by the clear transparent band development of zymogram stained with crystal violet, basic fuchsin, brilliant green and malachite green after polyacrylamide gel electrophoresis (PAGE) respectively. The decolorization enzyme was enzymologically characterized. The results showed that the molecular weight of TpmD is 29.4kDa and its isoelectric point (pI) is 5.6. The maximal activity of TpmD for above four triphenylmethane dyes was observed at 50 degrees C - 55 degrees C and pH 7.4 - 8.0. The temperature for losing half of the activity (t1/2) within 4h is 62 degrees C. The activities of decolorization enzyme are relatively stable at pH range of 5.5 - 9.0. The K(m) and V(max) of TpmD for decolorizing crystal violet, basic fuchsin, brilliant green and malachite green are 24.3, 40.6, 54.2, 68.5 micromol/L respectively, V(max) are 19.6, 74.1, 82.8, 115.6 micromol x L(-1) x s(-1) respectively. Both NADH/NADPH and molecular oxygen are necessary for the enzyme to decolorize triphenylmethane dyes, indicate the enzyme is an NADH/NADPH-dependent oxygenase.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-9
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
[Properties of a triphenylmethane dyes decolorization enzyme TpmD from Aeromonas hydrophila strain DN322].
pubmed:affiliation
Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China. rensz@163.com
pubmed:publicationType
Journal Article, English Abstract