| pubmed-article:1693161 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C0014833 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C0002199 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:1693161 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:1693161 | pubmed:dateCreated | 1990-7-3 | lld:pubmed |
| pubmed-article:1693161 | pubmed:abstractText | Recombinant plasmids for intracellular synthesis of mature porcine interferon alpha 1 (IFN-alpha 1) in Escherichia coli were constructed. High amounts of antiviral activity were obtained [up to 4 x 10(5) international units (IU) per ml of bacterial culture]. Recombinant porcine IFN-alpha 1 (rIFN-alpha 1) was purified to homogeneity by monoclonal antibody immunoaffinity and was found to have the expected Mr (17.5K) and N-terminal sequence (except for the apparent lack of an N-terminal methionine). Its specific antiviral activity was 5 x 10(7) to 10 x 10(7) IU/mg MDBK cells. In vitro biological properties of this purified rIFN-alpha 1 were compared to those of virus-induced porcine leukocyte interferon: the two interferons shared similar antigenic determinants and had the same ability to induce a cytocidal effect on primary cultures of pig kidney epithelial cells. However, rIFN-alpha 1 was at least six times more active in inducing an antiviral state on homologous porcine cells. These properties are discussed in the light of a possible in vivo use of the purified recombinant molecule. | lld:pubmed |
| pubmed-article:1693161 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1693161 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1693161 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1693161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1693161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1693161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1693161 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1693161 | pubmed:month | May | lld:pubmed |
| pubmed-article:1693161 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:1693161 | pubmed:author | pubmed-author:LefèvreFF | lld:pubmed |
| pubmed-article:1693161 | pubmed:author | pubmed-author:L'HaridonRR | lld:pubmed |
| pubmed-article:1693161 | pubmed:author | pubmed-author:La... | lld:pubmed |
| pubmed-article:1693161 | pubmed:author | pubmed-author:Borras-Cuesta... | lld:pubmed |
| pubmed-article:1693161 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1693161 | pubmed:volume | 71 ( Pt 5) | lld:pubmed |
| pubmed-article:1693161 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1693161 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1693161 | pubmed:pagination | 1057-63 | lld:pubmed |
| pubmed-article:1693161 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:meshHeading | pubmed-meshheading:1693161-... | lld:pubmed |
| pubmed-article:1693161 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1693161 | pubmed:articleTitle | Production, purification and biological properties of an Escherichia coli-derived recombinant porcine alpha interferon. | lld:pubmed |
| pubmed-article:1693161 | pubmed:affiliation | Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, France. | lld:pubmed |
| pubmed-article:1693161 | pubmed:publicationType | Journal Article | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1693161 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1693161 | lld:pubmed |
