Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1990-7-3
pubmed:abstractText
Recombinant plasmids for intracellular synthesis of mature porcine interferon alpha 1 (IFN-alpha 1) in Escherichia coli were constructed. High amounts of antiviral activity were obtained [up to 4 x 10(5) international units (IU) per ml of bacterial culture]. Recombinant porcine IFN-alpha 1 (rIFN-alpha 1) was purified to homogeneity by monoclonal antibody immunoaffinity and was found to have the expected Mr (17.5K) and N-terminal sequence (except for the apparent lack of an N-terminal methionine). Its specific antiviral activity was 5 x 10(7) to 10 x 10(7) IU/mg MDBK cells. In vitro biological properties of this purified rIFN-alpha 1 were compared to those of virus-induced porcine leukocyte interferon: the two interferons shared similar antigenic determinants and had the same ability to induce a cytocidal effect on primary cultures of pig kidney epithelial cells. However, rIFN-alpha 1 was at least six times more active in inducing an antiviral state on homologous porcine cells. These properties are discussed in the light of a possible in vivo use of the purified recombinant molecule.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
71 ( Pt 5)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1057-63
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Production, purification and biological properties of an Escherichia coli-derived recombinant porcine alpha interferon.
pubmed:affiliation
Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, France.
pubmed:publicationType
Journal Article