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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1990-7-3
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pubmed:abstractText |
Recombinant plasmids for intracellular synthesis of mature porcine interferon alpha 1 (IFN-alpha 1) in Escherichia coli were constructed. High amounts of antiviral activity were obtained [up to 4 x 10(5) international units (IU) per ml of bacterial culture]. Recombinant porcine IFN-alpha 1 (rIFN-alpha 1) was purified to homogeneity by monoclonal antibody immunoaffinity and was found to have the expected Mr (17.5K) and N-terminal sequence (except for the apparent lack of an N-terminal methionine). Its specific antiviral activity was 5 x 10(7) to 10 x 10(7) IU/mg MDBK cells. In vitro biological properties of this purified rIFN-alpha 1 were compared to those of virus-induced porcine leukocyte interferon: the two interferons shared similar antigenic determinants and had the same ability to induce a cytocidal effect on primary cultures of pig kidney epithelial cells. However, rIFN-alpha 1 was at least six times more active in inducing an antiviral state on homologous porcine cells. These properties are discussed in the light of a possible in vivo use of the purified recombinant molecule.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1317
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
71 ( Pt 5)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1057-63
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1693161-Animals,
pubmed-meshheading:1693161-Base Sequence,
pubmed-meshheading:1693161-Blotting, Western,
pubmed-meshheading:1693161-Cell Line,
pubmed-meshheading:1693161-Cell Survival,
pubmed-meshheading:1693161-Cytopathogenic Effect, Viral,
pubmed-meshheading:1693161-Epitopes,
pubmed-meshheading:1693161-Escherichia coli,
pubmed-meshheading:1693161-Humans,
pubmed-meshheading:1693161-Interferon Type I,
pubmed-meshheading:1693161-Molecular Sequence Data,
pubmed-meshheading:1693161-Plasmids,
pubmed-meshheading:1693161-Recombinant Proteins,
pubmed-meshheading:1693161-Swine,
pubmed-meshheading:1693161-Vesicular stomatitis Indiana virus
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pubmed:year |
1990
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pubmed:articleTitle |
Production, purification and biological properties of an Escherichia coli-derived recombinant porcine alpha interferon.
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pubmed:affiliation |
Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, France.
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pubmed:publicationType |
Journal Article
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