Source:http://linkedlifedata.com/resource/pubmed/id/16929843
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2006-8-25
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pubmed:abstractText |
A method has been developed for the determination of T-2 toxin in cereal grains by high performance liquid chromatography with fluorescence detection after immunoaffinity column clean-up and precolumn derivatization. The derivatization reaction was used to develop a sensitive, reproducible and accurate method for the determination of T-2 toxin in wheat, corn, barley and rice. T-2 toxin was extracted with methanol-water (80: 20, v/v), purified by immunoaffinity columns containing antibodies specific for T-2 toxin, and quantified by reversed-phase high performance liquid chromatography with fluorescence detection (excitation wavelength, 381 nm; emission wavelength, 470 nm) after derivatization with 1-anthroylnitrile (1-AN) and 4-dimethylaminopyridine (DMAP). ZORBAX Eclipse XDB-C18 column and mobile phase of acetonitrile-water (80: 20, v/v) were used for the analysis. Recoveries from the different cereals spiked with T-2 toxin at levels ranging from 0.01 to 1.5 microg/g were from 79.7% to 94.5%, the relative standard deviations were lower than 7% and the limit of detection was 0.01 microg/g based on a signal-to-noise ratio of 3: 1.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1000-8713
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
256-9
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pubmed:meshHeading |
pubmed-meshheading:16929843-Acetonitriles,
pubmed-meshheading:16929843-Animal Feed,
pubmed-meshheading:16929843-Cereals,
pubmed-meshheading:16929843-Chromatography, High Pressure Liquid,
pubmed-meshheading:16929843-Fluorescence,
pubmed-meshheading:16929843-Food Contamination,
pubmed-meshheading:16929843-Quality Control,
pubmed-meshheading:16929843-Spectrometry, Fluorescence,
pubmed-meshheading:16929843-T-2 Toxin
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pubmed:year |
2006
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pubmed:articleTitle |
[Determination of T-2 toxin in cereal grains by high performance liquid chromatography with fluorescence detection after immunoaffinity column clean-up and precolumn derivatization].
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pubmed:affiliation |
Liaoning Entry & Exit Inspection and Quarantine Bureau, Dalian, China. lj666623@sina.com
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pubmed:publicationType |
Journal Article,
English Abstract
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