Linked Life Data

16925600

Source:http://linkedlifedata.com/resource/pubmed/id/16925600

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-9-15
pubmed:abstractText
The expression profiles of Botrytis-inoculated Arabidopsis plants were studied to determine the nature of the defense transcriptome and to identify genes involved in host responses to the pathogen. Normally resistant Arabidopsis wild-type plants were compared with coi1, ein2, and nahG plants that are defective in various defense responses and/or show increased susceptibility to Botrytis. In wild-type plants, the expression of 621 genes representing approximately 0.48% of the Arabidopsis transcriptome was induced greater than or equal to twofold after infection. Of these 621 Botrytis-induced genes (BIGs), 462 were induced at or before 36 h post-inoculation, and may be involved in resistance to the pathogen. The expression of 181 BIGs was dependent on a functional COI1 gene required for jasmonate signaling, whereas the expression of 63 and 80 BIGs were dependent on ethylene (ET) signaling or salicylic acid accumulation, respectively, based on results from ein2 and nahG plants. BIGs encode diverse regulatory and structural proteins implicated in pathogen defense and abiotic and oxidative-stress responses. Thirty BIGs encode putative DNA-binding proteins that belong to ET response, zinc-finger, MYB, WRKY, and HD-ZIP family transcription-factor proteins. Fourteen BIGs were studied in detail to determine their role in resistance to Botrytis. T-DNA insertion alleles of ZFAR1 (At2G40140), the gene encoding a putative zinc-finger protein with ankyrin-repeat domains, showed increased local susceptibility to Botrytis and sensitivity to germination in the presence of abscisic acid (ABA), supporting the role of ABA in mediating responses to Botrytis infection. In addition, two independent T-DNA insertion alleles in the WRKY70 gene showed increased susceptibility to Botrytis. The transcriptional activation of genes involved in plant hormone signaling and synthesis, removal of reactive oxygen species, and defense and abiotic-stress responses, coupled with the susceptibility of the wrky70 and zfar1 mutants, highlights the complex genetic network underlying defense responses to Botrytis in Arabidopsis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-44
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16925600-Ankyrin Repeat, pubmed-meshheading:16925600-Arabidopsis, pubmed-meshheading:16925600-Arabidopsis Proteins, pubmed-meshheading:16925600-Botrytis, pubmed-meshheading:16925600-DNA-Binding Proteins, pubmed-meshheading:16925600-Ethylenes, pubmed-meshheading:16925600-Gene Expression Profiling, pubmed-meshheading:16925600-Gene Expression Regulation, Plant, pubmed-meshheading:16925600-Immunity, Innate, pubmed-meshheading:16925600-Mutagenesis, Insertional, pubmed-meshheading:16925600-Mutation, pubmed-meshheading:16925600-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16925600-Phenotype, pubmed-meshheading:16925600-Plant Diseases, pubmed-meshheading:16925600-Plant Leaves, pubmed-meshheading:16925600-Salicylic Acid, pubmed-meshheading:16925600-Transcription Factors, pubmed-meshheading:16925600-Zinc Fingers
pubmed:year
2006
pubmed:articleTitle
Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection.
pubmed:affiliation
Department of Botany and Plant Pathology, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.