Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-10-23
pubmed:abstractText
Mycobacterium tuberculosis is a highly successful human pathogen, with approximately 2x10(9) individuals infected globally. To understand the responses of M. tuberculosis to the in vivo environment, we studied the in vivo regulation of M. tuberculosis genes whose M. marinum homologs are induced in chronically infected frog tissues. The expression of 16S rRNA was shown to remain constant in M. tuberculosis under in vivo and in vitro conditions and therefore could be used for internal normalization in quantitative reverse transcription-PCR assays. We found whiB3, a putative transcriptional regulator implicated in mediating tissue damage, to be maximally induced at 2 weeks postinfection in the lungs of wild-type and immunodeficient (gamma interferon receptor-/-, Rag1-/-, and tumor necrosis factor alpha-/-) mice. At later time points in wild-type mice, whiB3 induction was decreased and gradually declined over the course of infection. In immunodeficient mice, whiB3 induction declined rapidly and was completely abolished in moribund animals. whiB3 was also found to be induced in naïve bone marrow-derived macrophages after 6 h of infection. whiB3 expression in vivo and in vitro was found to be inversely correlated with bacterial density. These results indicate that M. tuberculosis regulates the expression of whiB3 in response to environmental signals present in vivo and are consistent with a model of regulation by quorum sensing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-10827956, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-10835610, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-10934223, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-10963599, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11416222, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11489128, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11514514, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11880648, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11891270, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-11929527, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12065475, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12081975, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12123450, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12368434, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12479816, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12506197, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12897239, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12925133, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12953091, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-12953092, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-15070764, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-15618194, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-15928073, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-15939785, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-15955839, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-16497924, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-1904554, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-7504064, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-7540941, http://linkedlifedata.com/resource/pubmed/commentcorrection/16923787-8675308
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6449-57
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Regulation of Mycobacterium tuberculosis whiB3 in the mouse lung and macrophages.
pubmed:affiliation
Division of Infectious Diseases, Department of Medicine, New York University School of Medicine, Smilow Research Center, 522 First Avenue, SML 901, New York, NY 10016, USA. banain01@med.nyu.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural