Linked Life Data

1692315

Source:http://linkedlifedata.com/resource/pubmed/id/1692315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1990-6-13
pubmed:databankReference
pubmed:abstractText
The leukocyte adhesion molecule-1 (LAM-1, TQ1, Leu-8), expressed by human lymphocytes, neutrophils, monocytes, and their precursors, is a member of the selectin family of cellular adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. These cell surface molecules contain an amino-terminal lectin-like domain followed by an epidermal growth factor-like domain and a variable number of short consensus repeat sequences similar to those found in C3/C4 binding proteins. In this report, the structure of the lyam-1 gene that encodes the LAM-1 protein was determined by isolating overlapping genomic DNA clones that hybridized with a LAM-1 cDNA probe. The lyam-1 gene spans greater than 30 kilo base pairs of DNA and is composed of at least 10 exons. The 5' end of the LAM-1 mRNA was mapped by primer extension analysis revealing a single initiation region for transcription. Exons II through X contain translated sequences; exon II encodes the translation initiation codon; exon III, the leader peptide; IV, the lectin-like domain; V, the epidermal growth factor-like domain; VI and VII, the short consensus repeat units; exon VIII, the transmembrane region; exon IX encodes seven amino acids containing a potential phosphorylation site; and exon X encodes the five remaining amino acids of the cytoplasmic tail and the long 3' untranslated region. Sequencing of LAM-1 cDNA clones derived from neutrophils revealed that the protein expressed by neutrophils would be identical in sequence with the protein expressed by lymphocytes and cDNAs that would encode different isoforms of LAM-1 protein were not detected. In addition, the level of LAM-1 expression by lymphocytes and neutrophils from two patients with paroxysmal nocturnal hemoglobinuria, a disorder in which linkage of phosphatidylinositol anchors to proteins is defective, was similar to that of normal controls. Therefore, the usage of exons II through X results in the generation of a single major LAM-1 protein product expressed by lymphocytes and neutrophils.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7760-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1692315-Base Sequence, pubmed-meshheading:1692315-Cell Adhesion Molecules, pubmed-meshheading:1692315-Cloning, Molecular, pubmed-meshheading:1692315-Codon, pubmed-meshheading:1692315-DNA, pubmed-meshheading:1692315-DNA Probes, pubmed-meshheading:1692315-Exons, pubmed-meshheading:1692315-Gene Expression, pubmed-meshheading:1692315-Hemoglobinuria, Paroxysmal, pubmed-meshheading:1692315-Humans, pubmed-meshheading:1692315-Introns, pubmed-meshheading:1692315-L-Selectin, pubmed-meshheading:1692315-Lymphocytes, pubmed-meshheading:1692315-Molecular Sequence Data, pubmed-meshheading:1692315-Neutrophils, pubmed-meshheading:1692315-Nucleic Acid Hybridization, pubmed-meshheading:1692315-Polymerase Chain Reaction, pubmed-meshheading:1692315-Promoter Regions, Genetic, pubmed-meshheading:1692315-Protein Biosynthesis, pubmed-meshheading:1692315-Restriction Mapping
pubmed:year
1990
pubmed:articleTitle
Structure of the gene encoding the human leukocyte adhesion molecule-1 (TQ1, Leu-8) of lymphocytes and neutrophils.
pubmed:affiliation
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't