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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6270
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pubmed:dateCreated |
1990-5-31
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pubmed:databankReference |
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pubmed:abstractText |
Microbiologists have been constrained in their efforts to describe the compositions of natural microbial communities using traditional methods. Few microorganisms have sufficiently distinctive morphology to be recognized by microscopy. Culture-dependent methods are biased, as a microorganism can be cultivated only after its physiological niche is perceived and duplicated experimentally. It is therefore widely believed that fewer than 20% of the extant microorganisms have been discovered, and that culture methods are inadequate for studying microbial community composition. In view of the physiological and phylogenetic diversity among microorganisms, speculation that 80% or more of microbes remain undiscovered raises the question of how well we know the Earth's biota and its biochemical potential. We have performed a culture-independent analysis of the composition of a well-studied hot spring microbial community, using a common but distinctive cellular component, 16S ribosomal RNA. Our results confirm speculations about the diversity of uncultured microorganisms it contains.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-5
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1691827-Base Sequence,
pubmed-meshheading:1691827-Cloning, Molecular,
pubmed-meshheading:1691827-Cyanobacteria,
pubmed-meshheading:1691827-DNA,
pubmed-meshheading:1691827-Escherichia coli,
pubmed-meshheading:1691827-Eubacterium,
pubmed-meshheading:1691827-Euryarchaeota,
pubmed-meshheading:1691827-Fresh Water,
pubmed-meshheading:1691827-Hot Temperature,
pubmed-meshheading:1691827-Molecular Sequence Data,
pubmed-meshheading:1691827-Nucleic Acid Conformation,
pubmed-meshheading:1691827-Nucleic Acid Hybridization,
pubmed-meshheading:1691827-Phylogeny,
pubmed-meshheading:1691827-RNA, Bacterial,
pubmed-meshheading:1691827-RNA, Ribosomal,
pubmed-meshheading:1691827-RNA, Ribosomal, 16S,
pubmed-meshheading:1691827-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1691827-Water
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pubmed:year |
1990
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pubmed:articleTitle |
16S rRNA sequences reveal numerous uncultured microorganisms in a natural community.
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pubmed:affiliation |
Department of Microbiology, Montana State University, Bozeman 59717.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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