Source:http://linkedlifedata.com/resource/pubmed/id/16914663
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 9
|
| pubmed:dateCreated |
2006-8-17
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| pubmed:abstractText |
The in vivo contribution of reactive oxygen species produced by neutrophils against Cryptococcus infection is not widely recognized. Myeloperoxidase (MPO) is a neutrophil-specific enzyme that catalyses the production of hypohalous acids such as HOCl from H2O2. This study investigated the role of MPO in immunological defence against Cryptococcus neoformans in an MPO-deficient (MPO-/-) mouse model. The survival of MPO-/- mice infected either intranasally or intravenously with C. neoformans was lower than that of identically challenged wild-type mice. The MPO-/- mice that received intranasal injection of C. neoformans had significantly larger lung fungal burdens than wild-type mice. On day 7, MPO-/- mice had a significantly higher lung concentration of interleukin (IL)-4 and lower concentrations of IL-2, IL-12p70 and interferon (IFN)-gamma than wild-type mice, suggesting a weak Th1 response in the MPO-/- mice to C. neoformans. Pathologically, the MPO-/- mice with intranasal infection showed more severe pneumonia than wild-type mice, which was associated with an increase in the levels of IL-1alpha/beta in the lungs. In addition, in MPO-/- mice, the pulmonary infection disseminated to the brain with occasional meningitis. The keratinocyte-derived cytokine (KC) level in the brain of infected MPO-/- mice was higher than that of control mice. Both intranasal and intravenous infections resulted in a higher number of fungi in the spleen of MPO-/- mice compared to wild-type, suggesting decreased resistance to C. neoformans not only in the lungs but also in the spleen in the absence of MPO. Taken together, these data suggest a major role of MPO in the response to cryptococcal infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0022-2615
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
55
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1291-9
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:16914663-Animals,
pubmed-meshheading:16914663-Blood,
pubmed-meshheading:16914663-Brain,
pubmed-meshheading:16914663-Colony Count, Microbial,
pubmed-meshheading:16914663-Cryptococcosis,
pubmed-meshheading:16914663-Cryptococcus neoformans,
pubmed-meshheading:16914663-Cytokines,
pubmed-meshheading:16914663-Female,
pubmed-meshheading:16914663-Lung,
pubmed-meshheading:16914663-Meningitis, Cryptococcal,
pubmed-meshheading:16914663-Mice,
pubmed-meshheading:16914663-Mice, Knockout,
pubmed-meshheading:16914663-Neutrophils,
pubmed-meshheading:16914663-Peroxidase,
pubmed-meshheading:16914663-Pneumonia,
pubmed-meshheading:16914663-Reactive Oxygen Species,
pubmed-meshheading:16914663-Spleen,
pubmed-meshheading:16914663-Survival Analysis
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Contribution of the myeloperoxidase-dependent oxidative system to host defence against Cryptococcus neoformans.
|
| pubmed:affiliation |
Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka, Yokohama 244-0813, Japan. yaratani@yokohama-cu.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|