Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1990-5-24
|
| pubmed:abstractText |
Binding of the alpha-adrenergic antagonists [3H]prazosin and [3H]rauwolscine to well-characterized subcellular membrane fractions isolated from dog mesenteric arteries and veins was studied. Binding of both ligands was saturable with Kd values of 0.5 +/- 0.1 nM for [3H]prazosin and 5.85 +/- 0.85 nM for [3H]rauwolscine in arteries, and 0.87 +/- 0.4 nM for [3H]prazosin and 6.6 +/- 1.5 nM for [3H]rauwolscine in veins. In veins, the maximum number of binding sites for [3H]rauwolscine was higher than that for [3H]prazosin, whereas in arteries the maximum number of binding sites for each ligand was similar. In microsomes from dog aorta, the maximum number of bindings sites for [3H]prazosin was higher than that for [3H]rauwolscine. Neuronal membrane contamination in these studies was minimized by dissection procedures and evaluated by the comparison of [3H]saxitoxin binding in various preparations. Only mesenteric veins responded functionally to agonists acting on alpha 2 adrenoceptors. This study thus identified two distinct populations of [3H]prazosin and [3H]rauwolscine binding sites in the plasma membranes of dog mesenteric vessels and suggests that a much higher density of alpha 2-compared to alpha 1-adrenoceptor binding sites is required for a contractile response.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Electron Transport Complex IV,
http://linkedlifedata.com/resource/pubmed/chemical/N-Glycosyl Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH-Ferrihemoprotein Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Prazosin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Saxitoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/Yohimbine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0160-2446
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
515-26
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1691379-Animals,
pubmed-meshheading:1691379-Binding Sites,
pubmed-meshheading:1691379-Cell Membrane,
pubmed-meshheading:1691379-Dogs,
pubmed-meshheading:1691379-Electron Transport Complex IV,
pubmed-meshheading:1691379-Female,
pubmed-meshheading:1691379-Kinetics,
pubmed-meshheading:1691379-Male,
pubmed-meshheading:1691379-Mesenteric Arteries,
pubmed-meshheading:1691379-Mesenteric Veins,
pubmed-meshheading:1691379-Muscle, Smooth, Vascular,
pubmed-meshheading:1691379-N-Glycosyl Hydrolases,
pubmed-meshheading:1691379-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:1691379-Prazosin,
pubmed-meshheading:1691379-Radioligand Assay,
pubmed-meshheading:1691379-Receptors, Adrenergic, alpha,
pubmed-meshheading:1691379-Saxitoxin,
pubmed-meshheading:1691379-Tritium,
pubmed-meshheading:1691379-Yohimbine
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Alpha-adrenoceptors in dog mesenteric vessels--subcellular distribution and number of [3H]prazosin and [3H]rauwolscine binding sites.
|
| pubmed:affiliation |
Department of Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|