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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1990-5-17
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pubmed:abstractText |
Immunoblot analysis was used to characterize the Trichinella spiralis L1 larval Ag recognized by antisera from T. spiralis-infected AKR/J mice. Antisera were analyzed for reactivity with crude worm extract, excretory/secretory proteins and cuticle proteins from L1 larvae. The response was biphasic; antibodies against one set of Ag were detected 13 days after infection (group I Ag), and antibodies against a different set of Ag were detected 35 days after infection (group II Ag). Excretory/secretory and cuticle proteins were recognized only by antibodies produced late in infection. The predominant isotype in day 42 antiserum was IgG1, and 80% of these IgG1 antibodies reacted with a stage-specific determinant shared by virtually all group II Ag. A mAb reactive with the shared determinant was used to purify the group II Ag from L1 larval extract. Such affinity-purified Ag were protective when used to immunize mice against subsequent infection, and T cells from infected mice proliferated when cultured with these Ag in vitro. Other mouse strains also made a strong serum antibody response to the group II Ag. We hypothesize that immune responses to the shared epitope play an important role in determining the outcome of the host-parasite interaction during infection.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
144
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3152-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1691229-Administration, Oral,
pubmed-meshheading:1691229-Animals,
pubmed-meshheading:1691229-Antibodies, Helminth,
pubmed-meshheading:1691229-Antibody Specificity,
pubmed-meshheading:1691229-Antigens, Helminth,
pubmed-meshheading:1691229-Blotting, Western,
pubmed-meshheading:1691229-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:1691229-Epitopes,
pubmed-meshheading:1691229-Helminth Proteins,
pubmed-meshheading:1691229-Immunization, Passive,
pubmed-meshheading:1691229-Isoelectric Point,
pubmed-meshheading:1691229-Mice,
pubmed-meshheading:1691229-Mice, Inbred AKR,
pubmed-meshheading:1691229-Molecular Weight,
pubmed-meshheading:1691229-Time Factors,
pubmed-meshheading:1691229-Trichinella
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pubmed:year |
1990
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pubmed:articleTitle |
The mouse antibody response to Trichinella spiralis defines a single, immunodominant epitope shared by multiple antigens.
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pubmed:affiliation |
Department of Biochemistry, University of Wisconsin, Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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