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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-5-24
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pubmed:abstractText |
The protein and antigenic profiles of the American Type Culture Collection type strains of Mobiluncus species and those of 114 clinical isolates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and immunoblotting with homologous polyvalent antisera. The majority of isolates (82%) possessed characteristic protein profiles and could be identified to the species level by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The major protein bands were also antigenic, and some antigenic cross-reactivity was noted between the two Mobiluncus species. All of the isolates were examined for reactivity with a panel of 12 monoclonal antibodies previously prepared against the type strains. While 56 of 60 clinical isolates of Mobiluncus curtisii (93%) reacted with one or more of the monoclonal antibodies, only 23 of 54 clinical isolates which were identified as Mobiluncus mulieris by biochemical methods (48%) reacted with one or more of the monoclonal antibodies. One of the 4 M. curtisii isolates (25%) and 11 of the 31 M. mulieris isolates (35%) which did not react with the monoclonal antibodies also had atypical protein profiles. These results demonstrate a high degree of heterogeneity in the protein and antigenic profiles of Mobiluncus isolates and suggest that further taxonomic division may be appropriate.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-2914104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3068252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3090855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3262199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3323027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3501755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3526812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3585961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3924951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-3958220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6150262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6399410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6541662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6542001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6598924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691207-6598926
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1691207-Animals,
pubmed-meshheading:1691207-Antibodies, Monoclonal,
pubmed-meshheading:1691207-Antibody Specificity,
pubmed-meshheading:1691207-Antigens, Bacterial,
pubmed-meshheading:1691207-Bacteria, Anaerobic,
pubmed-meshheading:1691207-Bacterial Infections,
pubmed-meshheading:1691207-Bacterial Proteins,
pubmed-meshheading:1691207-Cross Reactions,
pubmed-meshheading:1691207-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1691207-Epitopes,
pubmed-meshheading:1691207-Female,
pubmed-meshheading:1691207-Fluorescent Antibody Technique,
pubmed-meshheading:1691207-Humans,
pubmed-meshheading:1691207-Immune Sera,
pubmed-meshheading:1691207-Immunoblotting,
pubmed-meshheading:1691207-Male,
pubmed-meshheading:1691207-Rabbits,
pubmed-meshheading:1691207-Species Specificity,
pubmed-meshheading:1691207-Vaginitis
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pubmed:year |
1990
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pubmed:articleTitle |
Demonstration of heterogeneity among the antigenic proteins of Mobiluncus species.
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pubmed:affiliation |
Department of Medicine, University of Washington, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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